Product nameAnti-GRB2 antibody [GRB-232]
See all GRB2 primary antibodies
DescriptionMouse monoclonal [GRB-232] to GRB2
Tested applicationsSuitable for: IP, WB, ELISA, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus monkey
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. Whole extract of cultured human acute T cell leukemia Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from culture supernatant of hybridoma cells.
Our Abpromise guarantee covers the use of ab49876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localizationGolgi apparatus.
- Information by UniProt
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
This product has been referenced in:
- Fritsche L et al. Insulin-induced serine phosphorylation of IRS-2 via ERK1/2 and mTOR: studies on the function of Ser675 and Ser907. Am J Physiol Endocrinol Metab 300:E824-36 (2011). IP ; Human . Read more (PubMed: 21098738) »
- Chentouf M et al. Excessive food intake, obesity and inflammation process in Zucker fa/fa rat pancreatic islets. PLoS One 6:e22954 (2011). WB ; Rat . Read more (PubMed: 21826222) »