Key features and details
- Mouse monoclonal [M331] to GRB2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-GRB2 antibody [M331]
See all GRB2 primary antibodies
DescriptionMouse monoclonal [M331] to GRB2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
Recombinant full length Rat protein
- PC12 cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab86713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 24 kDa.|
FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
Cellular localizationGolgi apparatus.
- Information by UniProt
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
Lane 1 : Anti-GRB2 antibody [M331] (ab86713) at 1/250 dilution
Lane 2 : Anti-GRB2 antibody [M331] (ab86713) at 1/500 dilution
Lane 3 : Anti-GRB2 antibody [M331] (ab86713) at 1/1000 dilution
All lanes : PC12 cell lysate
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab86713 has been referenced in 1 publication.
- Spangle JM & Munger K The HPV16 E6 oncoprotein causes prolonged receptor protein tyrosine kinase signaling and enhances internalization of phosphorylated receptor species. PLoS Pathog 9:e1003237 (2013). WB ; Human . PubMed: 23516367