Anti-GRB2 antibody [Y237] (ab32037)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-GRB2 antibody [Y237]
See all GRB2 primary antibodies -
DescriptionRabbit monoclonal [Y237] to GRB2
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK293, PC-12 and NIH/3T3 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberY237
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| IP | 1/50. | |
| IHC-P | 1/100 - 1/250. | |
| ICC/IF | 1/50 - 1/100. |
Target
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FunctionAdapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similaritiesBelongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
DomainThe SH3 domains mediate interaction with RALGPS1 and SHB.
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Cellular localizationGolgi apparatus.
- Information by UniProt
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Database links
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
- SwissProt: P62994 Rat
- Unigene: 444356 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-257525-UntitledD.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) cell lysate
Lane 3 : HEK293 (human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking/Dilution buffer : 5% NFDM/TBST
Exposure time : 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037IHCpara.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037. -
![Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037IFA.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)Immunofluorescent analysis of GRB2 expression in HeLa cells using 1/50 ab32037. -
![Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-287292-ab32037FC1.jpg)
Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GRB2 (red) with ab32037 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/GRB2-Primary-antibodies-ab32037-3.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)This image is a courtesy of Anonymous AbreviewAnti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/GRB2-Primary-antibodies-ab32037-5.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution +Recombinant human AHA1 protein (ab80352) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 2 minutes
Datasheets and documents
References
This product has been referenced in:
- Chang PM et al. Fucoidan?Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3ß/CREB Pathways and Metabolic Pathways in Senescent Mice. Mar Drugs 17:N/A (2019). Read more (PubMed: 30669571) »
- Arbulo-Echevarria MM et al. A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling. Front Immunol 9:115 (2018). Read more (PubMed: 29456532) »
