Recombinant Anti-GRB2 antibody [Y237] (ab32037)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-GRB2 antibody [Y237]
See all GRB2 primary antibodies -
Description
Rabbit monoclonal [Y237] to GRB2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GRB2 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK293, PC-12 and NIH/3T3 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y237 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| IP | 1/50. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
Sequence similarities
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The SH3 domains mediate interaction with RALGPS1 and SHB. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
- SwissProt: Q60631 Mouse
- SwissProt: P62994 Rat
- Unigene: 444356 Human
see all -
Alternative names
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-349908-anti-grb2-antibody-Y237-immunohistochemistry-heLa-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y237] (ab32037)Immunocytochemistry/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) cells staining GRB2 with ab32037 at 1/200 dilution (green), followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution (blue). Confocal image showing nuclear and weak cytoplasmic staining in HeLa cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution (red). DAPI was used as a nuclear counterstain.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is [secondary antibody name] ab150077 at 1/1000 dilution.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-257525-anti-grb2-antibody-y237-western-blot.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)All lanes : Anti-GRB2 antibody [Y237] (ab32037) at 1/10000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) cell lysate
Lane 3 : HEK293 (human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaBlocking/Dilution buffer : 5% NFDM/TBST
Exposure time : 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-43704-anti-grb2-antibody-y237-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y237] (ab32037)Immunohistochemical analysis of GRB2 expression in paraffin embedded human ovarian carcinoma, using 1/100 ab32037.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-287292-anti-grb2-antibody-y237-flow-cytometry.jpg)
Flow Cytometry - Anti-GRB2 antibody [Y237] (ab32037)Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GRB2 (red) with ab32037 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-43702-anti-grb2-antibody-y237-western-blot.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)This image is a courtesy of Anonymous AbreviewAnti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution + Lysate prepared from human Mesothelial cell line MeT-5A at 4 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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![Western blot - Anti-GRB2 antibody [Y237] (ab32037)](https://www.abcam.com/ps/products/32/ab32037/Images/ab32037-43701-anti-grb2-antibody-y237-western-blot.jpg)
Western blot - Anti-GRB2 antibody [Y237] (ab32037)Anti-GRB2 antibody [Y237] (ab32037) at 1/5000 dilution +Recombinant Human GRB2 protein (ab85917) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 2 minutes
Datasheets and documents
References
This product has been referenced in:
- Chang PM et al. Fucoidan?Fucoxanthin Ameliorated Cardiac Function via IRS1/GRB2/ SOS1, GSK3ß/CREB Pathways and Metabolic Pathways in Senescent Mice. Mar Drugs 17:N/A (2019). Read more (PubMed: 30669571) »
- Duggal S et al. Outlining the Grb2 interactome data and its interacting partners in HEK293 cells in absence and presence of epidermal growth factor. Data Brief 25:104082 (2019). Read more (PubMed: 31245515) »
