Recombinant
RabMAb

Anti-GRB2 antibody [Y301] (ab32111)

Abreviews (1)Q&A (2)Specific References (1)

Overview

  • Product name
    Anti-GRB2 antibody [Y301]
    See all GRB2 primary antibodies
  • Description
    Rabbit monoclonal [Y301] to GRB2
  • Host species
    Rabbit
  • Specificity
    ab32111 recognises GRB2. This antibody can also detect the splice isoform GRB3-3 of GRB2.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GRB2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • Human breast carcinoma, NIH3T3 cells, PC12 cells, HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
      Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
    • Sequence similarities
      Belongs to the GRB2/sem-5/DRK family.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Domain
      The SH3 domains mediate interaction with RALGPS1 and SHB.
    • Cellular localization
      Golgi apparatus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Abundant SRC homology antibody
      • Adapter protein GRB2 antibody
      • ASH antibody
      • Ash protein antibody
      • EGFRBP GRB2 antibody
      • Epidermal growth factor receptor binding protein antibody
      • Epidermal growth factor receptor binding protein GRB2 antibody
      • GRB 2 antibody
      • GRB2 adapter protein antibody
      • Grb2 antibody
      • GRB2_HUMAN antibody
      • Grb3 3 antibody
      • Growth factor receptor bound protein 2 antibody
      • Growth factor receptor bound protein 3 antibody
      • Growth factor receptor-bound protein 2 antibody
      • HT027 antibody
      • MST084 antibody
      • MSTP084 antibody
      • NCKAP2 antibody
      • OTTHUMP00000166096 antibody
      • OTTHUMP00000166097 antibody
      • OTTHUMP00000166098 antibody
      • Protein ash antibody
      • SEM5 antibody
      • SH2/SH3 adapter GRB2 antibody
      see all

    Images

    • Western blot - Anti-GRB2 antibody [Y301] (ab32111)
      Western blot - Anti-GRB2 antibody [Y301] (ab32111)
      All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/2000 dilution

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : HeLa cell lysate

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] (ab32111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] (ab32111)
      Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111.
    • Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y301] (ab32111)
      Immunocytochemistry/ Immunofluorescence - Anti-GRB2 antibody [Y301] (ab32111)
      Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
    • Flow Cytometry - Anti-GRB2 antibody [Y301] (ab32111)
      Flow Cytometry - Anti-GRB2 antibody [Y301] (ab32111)
      Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32111, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:
    • Parodi B  et al. Fumarates modulate microglia activation through a novel HCAR2 signaling pathway and rescue synaptic dysregulation in inflamed CNS. Acta Neuropathol 130:279-95 (2015). WB . Read more (PubMed: 25920452) »

    See 1 Publication for this product

    Publishing research using ab32111? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    3 reviews or Q&A

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    Votes: Highest First

    What is the recommended antigen retrieval buffer for these three antibodies?

    Thank you for contacting us.

    The following standard heat-mediated citrate buffer antigen retrieval was used for the IHC validation of ab32111 and ab111031. Antibody ab111586 used citrate buffer in a pressure cooker at 120C for 5 minutes but ...

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    What was the antigen retrieval method that was used to generate the picture on your datasheet?

    We used a heat mediated antigen retrieval method with sodium citrate, pH. 6.0.

    Western blot abreview for Anti-GRB2 antibody [Y301]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Raji)
    Loading amount
    40 µg
    Specification
    Raji
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    Submitted Sep 11 2009

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