Recombinant
RabMAb

Anti-GRB7 antibody [EPR4378] (ab109618)

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Overview

  • Product name
    Anti-GRB7 antibody [EPR4378]
    See all GRB7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4378] to GRB7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GRB7 aa 350-450. The exact sequence is proprietary.

  • Positive control
    • SKBR-3 and HeLa cell lysates. Human breast tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4378
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function
      Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.
    • Sequence similarities
      Belongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • B47 antibody
      • Epidermal growth factor receptor GRB 7 antibody
      • Epidermal growth factor receptor GRB-7 antibody
      • GRB7 adapter protein antibody
      • GRB7 antibody
      • GRB7_HUMAN antibody
      • Growth factor receptor bound protein 7 antibody
      • Growth factor receptor-bound protein 7 antibody
      • OTTHUMP00000164349 antibody
      • OTTHUMP00000164350 antibody
      • OTTHUMP00000164351 antibody
      • OTTHUMP00000164352 antibody
      see all

    Images

    • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
      ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    References

    ab109618 has not yet been referenced specifically in any publications.

    Publishing research using ab109618? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Could I please get the concentration for ab109618, lot GR48723-8?

    Thank you for contacting us.

    The concentration of lot GR48723-8 is 4.252 mg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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