Product nameAnti-GRB7 antibody [EPR4378]
See all GRB7 primary antibodies
DescriptionRabbit monoclonal [EPR4378] to GRB7
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GRB7 aa 350-450. The exact sequence is proprietary.
- WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 60 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat to 98°C, allow to cool for 10-20 minutes.
|ICC||1/50 - 1/100.|
FunctionInteracts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.
Sequence similaritiesBelongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
modificationsPhosphorylated on tyrosine residues.
- Information by UniProt
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)
Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.
ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa
ab109618 has not yet been referenced specifically in any publications.