Recombinant Anti-GRB7 antibody [EPR4378] (ab109618)
![Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)](https://www.abcam.com/ps/products/109/ab109618/Images/ab109618-382824-anti-grb7-antibody-epr4378-westernblot-human.jpg "Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4378] to GRB7
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-GRB7 antibody [EPR4378]
See all GRB7 primary antibodies -
Description
Rabbit monoclonal [EPR4378] to GRB7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4378 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/200 - 1/1000. Predicted molecular weight: 60 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat to 98°C, allow to cool for 10-20 minutes. |
| Notes |
|---|
|
WB
1/200 - 1/1000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat to 98°C, allow to cool for 10-20 minutes. |
Target
-
Function
Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. -
Sequence similarities
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues. - Information by UniProt
-
Database links
- Entrez Gene: 2886 Human
- Entrez Gene: 14786 Mouse
- Entrez Gene: 84427 Rat
- Omim: 601522 Human
- SwissProt: Q14451 Human
- SwissProt: Q03160 Mouse
- SwissProt: Q9QZC5 Rat
- Unigene: 86859 Human
see all -
Alternative names
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
see all
Images
-
Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)
Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)](https://www.abcam.com/ps/products/109/ab109618/Images/ab109618-122555-anti-grb7-antibody-epr4378-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)](https://www.abcam.com/ps/products/109/ab109618/Images/ab109618-122556-anti-grb7-antibody-epr4378-western-blot.jpg)
Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
![Anti-GRB7 antibody [EPR4378] (ab109618)](https://www.abcam.com/ps/products/109/ab109618/Images/ab109618-4-benefits-of-recombinant-antibodies.png)
Anti-GRB7 antibody [EPR4378] (ab109618)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab109618 has not yet been referenced specifically in any publications.