Recombinant
RabMAb

Recombinant Anti-GRB7 antibody [EPR4378] (ab109618)

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Overview

  • Product name

    Anti-GRB7 antibody [EPR4378]
    See all GRB7 primary antibodies

  • Description

    Rabbit monoclonal [EPR4378] to GRB7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GRB7 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4378

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat to 98°C, allow to cool for 10-20 minutes.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.

    • Sequence similarities

      Belongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine residues.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • B47 antibody
      • Epidermal growth factor receptor GRB 7 antibody
      • Epidermal growth factor receptor GRB-7 antibody
      • GRB7 adapter protein antibody
      • GRB7 antibody
      • GRB7_HUMAN antibody
      • Growth factor receptor bound protein 7 antibody
      • Growth factor receptor-bound protein 7 antibody
      • OTTHUMP00000164349 antibody
      • OTTHUMP00000164350 antibody
      • OTTHUMP00000164351 antibody
      • OTTHUMP00000164352 antibody
      see all

    Images

    • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)

      Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
      Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa



      Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

      The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)

      ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    References

    ab109618 has not yet been referenced specifically in any publications.

    Publishing research using ab109618? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Could I please get the concentration for ab109618, lot GR48723-8?

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    Answer

    Thank you for contacting us.

    The concentration of lot GR48723-8 is 4.252 mg/ml.

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