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Signal Transduction Adapters Cytoplasmic
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RecombinantRabMAb

Recombinant Anti-GRB7 antibody [EPR4378] (ab109618)

  • Datasheet
  • SDS
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Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
  • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
  • Anti-GRB7 antibody [EPR4378] (ab109618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4378] to GRB7
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-GRB7 antibody [EPR4378]
    See all GRB7 primary antibodies
  • Description

    Rabbit monoclonal [EPR4378] to GRB7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4378
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic

Associated products

  • Alternative Versions

    • Anti-GRB7 antibody [EPR4378] - BSA and Azide free (ab247908)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human GRB7 protein (ab125569)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/200 - 1/1000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat to 98°C, allow to cool for 10-20 minutes.

Notes
WB
1/200 - 1/1000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat to 98°C, allow to cool for 10-20 minutes.

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.
    • Sequence similarities

      Belongs to the GRB7/10/14 family.
      Contains 1 PH domain.
      Contains 1 Ras-associating domain.
      Contains 1 SH2 domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues.
    • Target information above from: UniProt accession Q14451 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2886 Human
      • Entrez Gene: 14786 Mouse
      • Entrez Gene: 84427 Rat
      • Omim: 601522 Human
      • SwissProt: Q14451 Human
      • SwissProt: Q03160 Mouse
      • SwissProt: Q9QZC5 Rat
      • Unigene: 86859 Human
      • Unigene: 276702 Mouse
      see all
    • Alternative names

      • B47 antibody
      • Epidermal growth factor receptor GRB 7 antibody
      • Epidermal growth factor receptor GRB-7 antibody
      • GRB7 adapter protein antibody
      • GRB7 antibody
      • GRB7_HUMAN antibody
      • Growth factor receptor bound protein 7 antibody
      • Growth factor receptor-bound protein 7 antibody
      • OTTHUMP00000164349 antibody
      • OTTHUMP00000164350 antibody
      • OTTHUMP00000164351 antibody
      • OTTHUMP00000164352 antibody
      see all

    Images

    • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)

      Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
      Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa



      Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

      The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)

      ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
      All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • Anti-GRB7 antibody [EPR4378] (ab109618)
      Anti-GRB7 antibody [EPR4378] (ab109618)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab109618? Please let us know so that we can cite the reference in this datasheet.

    ab109618 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Could I please get the concentration for ab109618, lot GR48723-8?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2013

    Answer

    Thank you for contacting us.

    The concentration of lot GR48723-8 is 4.252 mg/ml.

    Read More

    Elena Valued Customer

    Abcam Scientific Support

    Answered on Nov 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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