Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6858] to GRB7
- Suitable for: WB
- Reacts with: Human
Product nameAnti-GRB7 antibody [EPR6858]
See all GRB7 primary antibodies
DescriptionRabbit monoclonal [EPR6858] to GRB7
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human GRB7 (Q14451).
- SKBR-3 whole cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 60 kDa.|
FunctionInteracts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2.
Sequence similaritiesBelongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain.
modificationsPhosphorylated on tyrosine residues.
- Information by UniProt
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
All lanes : Anti-GRB7 antibody [EPR6858] (ab129091) at 1/1000 dilution
Lanes 1 & 5 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: Lane 1-4: 180 seconds; Lane 5: 7 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The GRB7 expression level in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID 17916906 and 7907978.
This image is derived from purified batch.
ab129091 has not yet been referenced specifically in any publications.