Anti-GRC5 / PHF2 antibody (ab65771)

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Overview

  • Product name

    Anti-GRC5 / PHF2 antibody
    See all GRC5 / PHF2 primary antibodies

  • Description

    Rabbit polyclonal to GRC5 / PHF2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

  • Positive control

    • Nuclear extracts of HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
IP Use at an assay dependent dilution.

Target

  • Function

    Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

  • Tissue specificity

    Widely expressed, including in liver (at protein level).

  • Sequence similarities

    Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.

  • Domain

    The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.

  • Post-translational
    modifications

    Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.

  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CENP-35 antibody
      • GRC5 antibody
      • JHDM1E antibody
      • Jumonji C domain containing histone demethylase 1E antibody
      • Lysine-specific demethylase PHF2 antibody
      • PHD finger protein 2 antibody
      • Phf2 antibody
      • PHF2_HUMAN antibody
      see all

    Images

    References

    ab65771 has not yet been referenced specifically in any publications.

    Publishing research using ab65771? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hi,
    We are interested in purchasing a PHF2 antibody. Your antibody ab65771
    seems to be promising, but we have some questions about the peptide used
    to produce the antibody. The issues are, a 269 aa peptide is very very
    big for a synthetic peptide; is the information online correct? And,
    both human and mouse PHF2 have 1096 amino acids, while on the
    information on your webpage indicates the synthetic peptide used to
    generate the ab "corresponding to C terminal amino acids 830-1098 of
    Human GRC5 / PHF2". If possible please let us know if any of these
    information is incorrect, and provide us the correct information.
    Thank you,

    Read More
    Answer

    Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
    You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 1096. It may be more accurate to call this a fragment than a peptide.
    I have updated the datasheet with the correct immunogen information, but please let me know if you have any further questions or if there is anything else that we can do for you.
    Have a nice day!

    Read More

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