Anti-GRC5 / PHF2 antibody (ab65771)
Key features and details
- Rabbit polyclonal to GRC5 / PHF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRC5 / PHF2 antibody
See all GRC5 / PHF2 primary antibodies -
Description
Rabbit polyclonal to GRC5 / PHF2 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2
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Positive control
- Nuclear extracts of HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa). |
Target
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Function
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. -
Tissue specificity
Widely expressed, including in liver (at protein level). -
Sequence similarities
Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger. -
Domain
The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3. -
Post-translational
modificationsPhosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 5253 Human
- Omim: 604351 Human
- SwissProt: O75151 Human
- Unigene: 211441 Human
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Alternative names
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
see all
Images
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Anti-GRC5 / PHF2 antibody (ab65771) at 1/1000 dilution + Nuclear extracts of HeLa cells.
Predicted band size: 121 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (0)
ab65771 has not yet been referenced specifically in any publications.