Anti-GRC5 / PHF2 antibody (ab65771)

Submit a review Q&A (1)
Western blot - Anti-GRC5 / PHF2 antibody - Carboxyterminal end (ab65771)

    Key features and details

    • Rabbit polyclonal to GRC5 / PHF2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-GRC5 / PHF2 antibody
      See all GRC5 / PHF2 primary antibodies

    • Description

      Rabbit polyclonal to GRC5 / PHF2

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide, corresponding to C terminal amino acids 830-1096 of Human GRC5/ PHF2

    • Positive control

      • Nuclear extracts of HeLa cells.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab65771 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).

    Target

    • Function

      Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

    • Tissue specificity

      Widely expressed, including in liver (at protein level).

    • Sequence similarities

      Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
      Contains 1 JmjC domain.
      Contains 1 PHD-type zinc finger.

    • Domain

      The PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.

    • Post-translational
      modifications

      Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.

    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt

    • Database links

      • Alternative names

        • CENP-35 antibody
        • GRC5 antibody
        • JHDM1E antibody
        • Jumonji C domain containing histone demethylase 1E antibody
        • Lysine-specific demethylase PHF2 antibody
        • PHD finger protein 2 antibody
        • Phf2 antibody
        • PHF2_HUMAN antibody
        see all

      Images

      References (0)

      Publishing research using ab65771? Please let us know so that we can cite the reference in this datasheet.

      ab65771 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hi,
      We are interested in purchasing a PHF2 antibody. Your antibody ab65771
      seems to be promising, but we have some questions about the peptide used
      to produce the antibody. The issues are, a 269 aa peptide is very very
      big for a synthetic peptide; is the information online correct? And,
      both human and mouse PHF2 have 1096 amino acids, while on the
      information on your webpage indicates the synthetic peptide used to
      generate the ab "corresponding to C terminal amino acids 830-1098 of
      Human GRC5 / PHF2". If possible please let us know if any of these
      information is incorrect, and provide us the correct information.
      Thank you,

      Read More
      Answer

      Thank you for contacting us and for your patience while I have been in touch with the lab regarding your enquiry.
      You are correct that there was a mistake on the datasheet. The immunogen runs from amino acid 830 to the C-terminus at amino acid 1096. It may be more accurate to call this a fragment than a peptide.
      I have updated the datasheet with the correct immunogen information, but please let me know if you have any further questions or if there is anything else that we can do for you.
      Have a nice day!

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com