Product nameAnti-GRC5 / PHF2 antibody
See all GRC5 / PHF2 primary antibodies
DescriptionRabbit polyclonal to GRC5 / PHF2
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 650-700 of Human GRC5/ PHF2 using the numbering given in entry NP_005383.3
- HeLa and 293T whole cell cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab123507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 120 kDa.|
FunctionLysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
Tissue specificityWidely expressed, including in liver (at protein level).
Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
modificationsPhosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
All lanes : Anti-GRC5 / PHF2 antibody (ab123507) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa at 15 µg
Lane 2 : Whole cell lysate from HeLa at 50 µg
Lane 3 : Whole cell lysate from 293T at 50 µg
Lane 4 : Whole cell lysate from Jurkat at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 30 seconds
ab123507 has not yet been referenced specifically in any publications.