Product nameAnti-GRC5 / PHF2 antibody
See all GRC5 / PHF2 primary antibodies
DescriptionRabbit polyclonal to GRC5 / PHF2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human GRC5/ PHF2.
- This antibody gave a positive signal in both Human and Mouse Liver tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 120 kDa).|
FunctionLysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
Tissue specificityWidely expressed, including in liver (at protein level).
Sequence similaritiesBelongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
Contains 1 JmjC domain.
Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger mediates the binding to H3K4me2 and H3K4me3.
modificationsPhosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- CENP-35 antibody
- GRC5 antibody
- JHDM1E antibody
All lanes : Anti-GRC5 / PHF2 antibody (ab124434) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The predicted molecular weight of GRC5 / PHF2 is 120 kDa (SwissProt), however we expect to observe a banding pattern around 160 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124434 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab124434 has not yet been referenced specifically in any publications.