Product nameAnti-GREB1 antibody
See all GREB1 primary antibodies
DescriptionRabbit polyclonal to GREB1
Tested applicationsSuitable for: ICC/IF, WB, ChIP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human GREB1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human prostate tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab72999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 216 kDa (predicted molecular weight: 216 kDa).|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml.|
FunctionUnknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers.
Tissue specificityExpressed in proliferating prostatic tissue and prostate cancer.
Sequence similaritiesBelongs to the GREB1 family.
- Information by UniProt
- 5730583K22Rik antibody
- 9130004E13 antibody
- AF180470 antibody
Anti-GREB1 antibody (ab72999) at 1 µg/ml + Human Prostate Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 216 kDa
Observed band size: 216 kDa
Exposure time: 2 minutes
ICC/IF image of ab72999 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of GREB1 antibody staining in a section of formalin-fixed paraffin-embedded normal human prostate performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab72999, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab72999 has been referenced in 5 publications.
- Savitski MM et al. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell 173:260-274.e25 (2018). PubMed: 29551266
- Wu Y et al. Tamoxifen Resistance in Breast Cancer Is Regulated by the EZH2-ERa-GREB1 Transcriptional Axis. Cancer Res 78:671-684 (2018). PubMed: 29212856
- Zhang P et al. Endometrial cancer-associated mutants of SPOP are defective in regulating estrogen receptor-a protein turnover. Cell Death Dis 6:e1687 (2015). PubMed: 25766326
- Spanheimer PM et al. Inhibition of RET increases the efficacy of antiestrogen and is a novel treatment strategy for luminal breast cancer. Clin Cancer Res 20:2115-25 (2014). WB . PubMed: 24526731
- Mohammed H et al. Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor. Cell Rep 3:342-9 (2013). ChIP ; Human . PubMed: 23403292