Product nameAnti-GRF2 antibody
See all GRF2 primary antibodies
DescriptionRabbit polyclonal to GRF2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Orangutan, Bat
Synthetic peptide corresponding to a region between residues 50 and 100 of human GRF2 (NP_005303.2)
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 121 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionGuanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
Tissue specificityUbiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Sequence similaritiesContains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.
- Information by UniProt
FormIt localizes to cytoplasm and endosomes.
- C3G antibody
- C3G protein antibody
- CRK SH3 binding GNRP antibody
All lanes : Anti-GRF2 antibody (ab86224) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Exposure time: 3 minutes
Detection of human GRF2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86224 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab86224 has not yet been referenced specifically in any publications.