Key features and details
- Rabbit polyclonal to GRF2 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRF2 antibody - C-terminal
See all GRF2 primary antibodies
DescriptionRabbit polyclonal to GRF2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GRF2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q13905
- WB: K562, THP-1 and HL-60 whole cell extracts.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 121 kDa.|
FunctionGuanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.
Tissue specificityUbiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Sequence similaritiesContains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.
- Information by UniProt
FormIt localizes to cytoplasm and endosomes.
- C3G antibody
- C3G protein antibody
- CRK SH3 binding GNRP antibody
All lanes : Anti-GRF2 antibody - C-terminal (ab229730) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 121 kDa
5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229730 has not yet been referenced specifically in any publications.