Anti-GRHL2 antibody (ab88631)
Key features and details
- Mouse polyclonal to GRHL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRHL2 antibody
See all GRHL2 primary antibodies -
Description
Mouse polyclonal to GRHL2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GRHL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- A431 cell lysate Transfected 293T cell lysate HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May function as a transcription factor. -
Tissue specificity
Highly expressed in placenta, prostate, brain and kidney. Lower-level expression in a variety of epithelial tissues such as thymus, kidney, lung, salivary gland, mammary gland and digestive tract. Expressed in the cochlear. -
Involvement in disease
Defects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28) [MIM:608641]. DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age. -
Sequence similarities
Belongs to the grh/CP2 family. Grainyhead subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79977 Human
- Omim: 608576 Human
- SwissProt: Q6ISB3 Human
- Unigene: 661088 Human
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Alternative names
- BOM antibody
- Brother of mammalian grainyhead antibody
- Deafness autosomal dominant 28 antibody
see all
Images
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Anti-GRHL2 antibody (ab88631) at 1 µg/ml + A431 cell lysate at 50 µg/ml
Predicted band size: 71 kDa -
All lanes : Anti-GRHL2 antibody (ab88631) at 1 µg/ml
Lane 1 : GRHL2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 71 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells using ab88631 at a dilution of 10 µg/ml followed by fluorescein conjugated secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88631 has been referenced in 2 publications.
- Chen L et al. Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Sci Rep 8:15543 (2018). PubMed: 30341388
- Fishwick C et al. Heterarchy of transcription factors driving basal and luminal cell phenotypes in human urothelium. Cell Death Differ 24:809-818 (2017). PubMed: 28282036