Key features and details
- Mouse polyclonal to GRHL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRHL2 antibody
See all GRHL2 primary antibodies
DescriptionMouse polyclonal to GRHL2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human protein (NP_079191.1)
- A431 cell lysate Transfected 293T cell lysate HeLa cells
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionMay function as a transcription factor.
Tissue specificityHighly expressed in placenta, prostate, brain and kidney. Lower-level expression in a variety of epithelial tissues such as thymus, kidney, lung, salivary gland, mammary gland and digestive tract. Expressed in the cochlear.
Involvement in diseaseDefects in GRHL2 are the cause of deafness autosomal dominant type 28 (DFNA28) [MIM:608641]. DFNA28 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA28 is characterized by mild to moderate hearing loss across most frequencies that progressed to severe loss in the higher frequencies by the fifth decade. Age at onset varied, with the earliest case documented at 7 years of age.
Sequence similaritiesBelongs to the grh/CP2 family. Grainyhead subfamily.
- Information by UniProt
- BOM antibody
- Brother of mammalian grainyhead antibody
- Deafness autosomal dominant 28 antibody
Anti-GRHL2 antibody (ab88631) at 1 µg/ml + A431 cell lysate at 50 µg/ml
Predicted band size: 71 kDa
All lanes : Anti-GRHL2 antibody (ab88631) at 1 µg/ml
Lane 1 : GRHL2-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 71 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of HeLa cells using ab88631 at a dilution of 10 µg/ml followed by fluorescein conjugated secondary antibody.
ab88631 has been referenced in 2 publications.
- Chen L et al. Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Sci Rep 8:15543 (2018). PubMed: 30341388
- Fishwick C et al. Heterarchy of transcription factors driving basal and luminal cell phenotypes in human urothelium. Cell Death Differ 24:809-818 (2017). PubMed: 28282036