Anti-GRHL3 antibody (ab221058)
Key features and details
- Rabbit polyclonal to GRHL3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GRHL3 antibody
See all GRHL3 primary antibodies -
Description
Rabbit polyclonal to GRHL3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human GRHL3 aa 50-150.
Database link: Q8TE85 -
Positive control
- IHC-P: Human urinary bladder, skin and oral mucosa tissue. ICC: MCF7 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
May function as a transcription factor. -
Tissue specificity
Expressed in brain, colon, pancreas, placenta and kidney. Isoform 1 is expressed in lung and tonsil. Isoform 2 is prostate-specific. -
Sequence similarities
Belongs to the grh/CP2 family. Grainyhead subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57822 Human
- Omim: 608317 Human
- SwissProt: Q8TE85 Human
- Unigene: 657920 Human
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Alternative names
- Drosophila antibody
- Grainyhead like 3 antibody
- Grainyhead like protein 3 homolog antibody
see all
Images
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Immunohistochemical analysis of human urinary bladder tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling GRHL3 (green) in the nucleoplasm with ab221058 at 2 µg/mL.
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Immunohistochemical analysis of human skin tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human oral mucosa tissue labeling GRHL3 in the nucleus of squamous epithelial cells with ab221058 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human skeletal muscle tissue labeling GRHL3 with ab221058 at a 1/1000 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221058 has not yet been referenced specifically in any publications.