Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
FunctionMay function as a transcription factor.
Tissue specificityExpressed in brain, colon, pancreas, placenta and kidney. Isoform 1 is expressed in lung and tonsil. Isoform 2 is prostate-specific.
Sequence similaritiesBelongs to the grh/CP2 family. Grainyhead subfamily.
- Information by UniProt
- Drosophila antibody
- Grainyhead like 3 antibody
- Grainyhead like protein 3 homolog antibody
Lane 1 : marker
Lane 2 : Anti-GRHL3 antibody (ab50755) at 0.5 µg/ml
Lane 2 : HepG2 cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50,000.
Predicted band size: 70 kDa
Observed band size: ~57 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab50755 has not yet been referenced specifically in any publications.