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Rabbit polyclonal to GRHL3
HumanPredicted to work with:
A region within synthetic peptide: SGVKGCLLSG FRGNETTYLR PETDLETPPV LFIPNVHFSS LQRSGGAAPS, corresponding to amino acids 428-477 of Human GRHL3
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 70 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
May function as a transcription factor.
Expressed in brain, colon, pancreas, placenta and kidney. Isoform 1 is expressed in lung and tonsil. Isoform 2 is prostate-specific.
Belongs to the grh/CP2 family. Grainyhead subfamily.
Information by UniProt
- Drosophila antibody
- Grainyhead like 3 antibody
- Grainyhead like protein 3 homolog antibody
Western blot - Anti-GRHL3 antibody (ab50755)
Lane 1 :
markerLane 2 :
Anti-GRHL3 antibody (ab50755) at 0.5 µg/mlLane 2 :
HepG2 cell lysate at 10 µgSecondaryLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50,000.Predicted band size:
70 kDaObserved band size:
~57 kDa (why is the actual band size different from the predicted?
Gel concentration 12%
has not yet been referenced specifically in any publications.
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