Key features and details
- Rabbit polyclonal to GRHPR
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GRHPR antibody
See all GRHPR primary antibodies
DescriptionRabbit polyclonal to GRHPR
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human GRHPR aa 1-328.
MRPVRLMKVFVTRRIPAEGRVALARAADCEVEQWDSDEPIPAKELERGVA GAHGLLCLLSDHVDKRILDAAGANLKVISTMSVGIDHLALDEIKKRGIRV GYTPDVLTDTTAELAVSLLLTTCRRLPEAIEEVKNGGWTSWKPLWLCGYG LTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRPEEAAEFQAEFV STPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINISRGDVVN QDDLYQALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHR TRNTMSLLAANNLLAGLRGEPMPSELKL
Database link: Q9UBQ7
- WB: Rat and mouse liver lysate. IHC-P: Human liver and testis tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 36 kDa.|
FunctionEnzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate.
Tissue specificityUbiquitous. Most abundantly expressed in the liver.
Involvement in diseaseDefects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2) [MIM:260000]; also known as primary hyperoxaluria type II (PH2). HP2 is a disorder where the main clinical manifestation is calcium oxalate nephrolithiasis though chronic as well as terminal renal insufficiency has been described. It is characterized by an elevated urinary excretion of oxalate and L-glycerate.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
- Information by UniProt
- GLXR antibody
- glycerate 2 dehydrogenase antibody
- GLYD antibody
All lanes : Anti-GRHPR antibody (ab236665) at 1/500 dilution
Lane 1 : Rat liver lysate
Lane 2 : Mouse liver lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Paraffin-embedded human liver tissue stained for GRHPR using ab236665 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for GRHPR using ab236665 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling GRHPR using ab236665 at 1/100 dilution in ICC/IF. Secondary antibody was Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236665 has not yet been referenced specifically in any publications.