Recombinant
RabMAb

Recombinant Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)

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Overview

  • Product name

    Anti-GRID2 antibody [EPR15422] - N-terminal
    See all GRID2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15422] to GRID2 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GRID2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O43424

  • Positive control

    • Rat brain, Mouse brain, Human cerebellum, Human fetal brain and HeLa cell lysates.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function

    Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.

  • Domain

    The PDZ-binding motif mediates interaction with GOPC.

  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • delta-2 antibody
    • GluD2 antibody
    • GLUR D2 antibody
    • GluR delta 2 antibody
    • GluR delta-2 subunit antibody
    • GLURD 2 antibody
    • GLURD2 antibody
    • Glutamate receptor delta 2 subunit antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor ionotropic delta 2 antibody
    • GRID 2 antibody
    • Grid2 antibody
    • GRID2_HUMAN antibody
    • MGC117022 antibody
    • MGC117023 antibody
    • MGC117024 antibody
    see all

Images

  • Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    All lanes : Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
    Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 113 kDa
    Observed band size: 113 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab198499 has not yet been referenced specifically in any publications.

Publishing research using ab198499? Please let us know so that we can cite the reference in this datasheet.

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