Recombinant Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
![Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)](https://www.abcam.com/ps/products/198/ab198499/Images/ab198499-239979-anti-grid2-antibody-epr15422-n-terminal-western-blot.jpg "Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15422] to GRID2 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GRID2 antibody [EPR15422] - N-terminal
See all GRID2 primary antibodies -
Description
Rabbit monoclonal [EPR15422] to GRID2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GRID2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O43424 -
Positive control
- Rat brain, Mouse brain, Human cerebellum, Human fetal brain and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15422 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab198499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
Target
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Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily. -
Domain
The PDZ-binding motif mediates interaction with GOPC. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2895 Human
- Entrez Gene: 14804 Mouse
- Entrez Gene: 79220 Rat
- Omim: 602368 Human
- SwissProt: O43424 Human
- SwissProt: Q61625 Mouse
- SwissProt: Q63226 Rat
- Unigene: 480281 Human
see all -
Alternative names
- delta-2 antibody
- GluD2 antibody
- GLUR D2 antibody
see all
Images
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Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)All lanes : Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)](https://www.abcam.com/ps/products/198/ab198499/Images/ab198499-239980-anti-grid2-antibody-epr15422-n-terminal-western-blot.jpg)
Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human fetal brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)](https://www.abcam.com/ps/products/198/ab198499/Images/ab198499-239981-anti-grid2-antibody-epr15422-n-terminal-western-blot.jpg)
Western blot - Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)](https://www.abcam.com/ps/products/198/ab198499/Images/ab198499-4-benefits-of-recombinant-antibodies.png)
Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
Datasheets and documents
Certificate of Compliance
References (0)
ab198499 has not yet been referenced specifically in any publications.
