Recombinant Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15422] to GRID2 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GRID2 antibody [EPR15422] - N-terminal
See all GRID2 primary antibodies -
Description
Rabbit monoclonal [EPR15422] to GRID2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat brain, Mouse brain, Human cerebellum, Human fetal brain and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15422 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
Target
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Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily. -
Domain
The PDZ-binding motif mediates interaction with GOPC. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2895 Human
- Entrez Gene: 14804 Mouse
- Entrez Gene: 79220 Rat
- Omim: 602368 Human
- SwissProt: O43424 Human
- SwissProt: Q61625 Mouse
- SwissProt: Q63226 Rat
- Unigene: 480281 Human
see all -
Alternative names
- delta-2 antibody
- GluD2 antibody
- GLUR D2 antibody
see all
Images
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Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/50000 dilution + Human cerebellum lysate (boiled) at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution
Lane 1 : Mouse brain lysate (boiled)
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate (boiled)
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 113 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-GRID2 antibody [EPR15422] - N-terminal (ab198499) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab198499 has been referenced in 1 publication.
- Lin H et al. Validation of the functions and prognostic values of synapse-associated proteins in lower-grade glioma. Biosci Rep 41:N/A (2021). PubMed: 33969375