Overview

  • Product name

    Griess reagent
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    1 nmol/well
  • Assay time

    1h 30m
  • Product overview

    Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.


    Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.


    The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 200 tests 500 tests 1000 tests
    Griess Reagent I 1 x 10ml 1 x 25ml 1 x 50ml
    Griess Reagent II 1 x 10ml 1 x 25ml 1 x 50ml
    Nitrite Assay Buffer 1 x 30ml 1 x 75ml 1 x 150ml
    Nitrite Standard 1 vial 1 vial 1 vial

Images

  • Human serum was deproteinized using 10 kDa columns. Filtrate (20 µl, undiluted) was collected and assayed according to kit protocols. HeLa nuclear cells were homogenized using Nitrite Assay Buffer (100 µg protein).

Protocols

References

ab234044 has not yet been referenced specifically in any publications.

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