Product nameAnti-GRIK2 + GRIK3 antibody
DescriptionRabbit polyclonal to GRIK2 + GRIK3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Chicken, Monkey
- Brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 105 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab15307 staining GRIK2/3 in human brain by Immunohistochemistry (FFPE-sections).
ab15307 at 1/200 staining rat brain (PAG) tissue sections by IHC (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed and the tissue was then incubated with the antibody for 24 hours. A biotinylated goat polyclonal antibody was used as the secondary.
Anti-GRIK2 + GRIK3 antibody (ab15307) at 1/2000 dilution + Rat brain cortex lysate at 15ug per lane
Horse anti-rabbit HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds