Key features and details
- Mouse monoclonal [8A1F11] to GRIK2/GluK2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-GRIK2/GluK2 antibody [8A1F11]
See all GRIK2/GluK2 primary antibodies
DescriptionMouse monoclonal [8A1F11] to GRIK2/GluK2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey
Recombinant fragment corresponding to Human GRIK2/GluK2 aa 45-226.
VESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKK ACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDS FYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSR YNLRLKIRQLPADTKDAKPLLKEMKRGKEFHV
Database link: Q13002
- WB: Recombinant human GRIK2/GluK2 (aa 45-226) protein; Human GRIK2/GluK2 (aa 45-226)-hIgGFc transfected HEK-293 cell lysate.
Previously labelled as GRIK2.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2.
Tissue specificityExpression is higher in cerebellum than in cerebral cortex.
Involvement in diseaseDefects in GRIK2 are the cause of mental retardation autosomal recessive type 6 (MRT6) [MIM:611092]. It is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic mental retardation. MRT6 patients display mild to severe mental retardation and psychomotor development delay in early childhood. Patients do not have neurologic problems, congenital malformations, or facial dysmorphism. Body height, weight, and head circumference are normal.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK2 subfamily.
modificationsSumoylation mediates kainate receptor-mediated endocytosis and regulates synaptic transmission. Sumoylation is enhanced by PIAS3 and desumoylated by SENP1.
Ubiquitinated. Ubiquitination regulates the GRIK2 levels at the synapse by leading kainate receptor degradation through proteasome.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- bA487F5.1 antibody
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Anti-GRIK2/GluK2 antibody [8A1F11] (ab233719) at 1/500 dilution + Recombinant human GRIK2/GluK2 (aa 45-226) protein
Developed using the ECL technique.
Expected MW is 47 kDa.
All lanes :
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Human GRIK2/GluK2 (aa 45-226)-hIgGFc transfected HEK-293 cell lysate
Developed using the ECL technique.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233719 has not yet been referenced specifically in any publications.