Key features and details
- Mouse monoclonal [2B3D1] to GRIK3/GluK3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-GRIK3/GluK3 antibody [2B3D1]
See all GRIK3/GluK3 primary antibodies
DescriptionMouse monoclonal [2B3D1] to GRIK3/GluK3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey
- WB: Human GRIK3/GluK3 (aa 32-173) recombinant protein; GRIK3/GluK3 (aa 32-173)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells.
This product was previously labelled as GRIK3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 104 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds domoate > kainate >> L-glutamate = quisqualate >> AMPA = NMDA.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK3 subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
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Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution + Human GRIK3/GluK3 (aa 32-173) recombinant protein.
Predicted band size: 104 kDa
Expected MW is 42 kDa.
All lanes : Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : GRIK3/GluK3 (aa 32-173)-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 104 kDa
Immunofluorescence analysis of Hela (human epithelial cell line from cervix adenocarcinoma) cells labelling GRIK3/GluK3 using ab233734 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments labeled with AlexaFluor®-555-Phalloidin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233734 has not yet been referenced specifically in any publications.