Anti-GRIM19 antibody [6E1BH7] (Alexa Fluor® 488) (ab197965)

Overview

  • Product name

    Anti-GRIM19 antibody [6E1BH7] (Alexa Fluor® 488)
    See all GRIM19 primary antibodies
  • Description

    Mouse monoclonal [6E1BH7] to GRIM19 (Alexa Fluor® 488)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Human GRIM19.

  • Positive control

    • ICC/IF: MEF1 cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab197965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in MEF1 cells fixed with 100% methanol (5 min).

Target

  • Function

    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.
  • Tissue specificity

    Widely expressed, with highest expression in heart, skeletal muscle, liver, kidney and placenta. In intestinal mucosa, down-regulated in areas involved in Crohn disease and ulcerative colitis.
  • Involvement in disease

    Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
  • Sequence similarities

    Belongs to the complex I NDUFA13 subunit family.
  • Developmental stage

    Expressed in numerous fetal tissues.
  • Cellular localization

    Mitochondrion inner membrane. Nucleus. May be translocated into the nucleus upon IFN/RA treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2700054G14Rik antibody
    • AU022060 antibody
    • B16.6 antibody
    • CDA016 antibody
    • Cell death regulatory protein antibody
    • Cell death regulatory protein GRIM-19 antibody
    • CGI-39 antibody
    • CGI39 protein antibody
    • CI-B16.6 antibody
    • Complex I-B16.6 antibody
    • FLJ58045 antibody
    • FLJ59191 antibody
    • Gene associated with retinoic and IFN-induced mortality 19 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 19 protein antibody
    • Gene associated with retinoic interferon induced mortality 19 protein antibody
    • GRIM-19 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 antibody
    • NADH ubiquinone oxidoreductase B16.6 subunit antibody
    • NADH-ubiquinone oxidoreductase B16.6 subunit antibody
    • NDUAD_HUMAN antibody
    • NDUFA13 antibody
    • RGD1565358 antibody
    see all

Images

  • ab197965 staining GRIM19 in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197965 at a 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab197965 has not yet been referenced specifically in any publications.

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