Anti-GRIP1 antibody (ab168848)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GRIP1 antibody
See all GRIP1 primary antibodies -
Description
Rabbit polyclonal to GRIP1 -
Host species
Rabbit -
Specificity
This antibody is raised against glutamate receptor-interacting protein 1 -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 725-775 of Human GRIP1 (NP_066973.2).
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Positive control
- 293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab168848 was affinity purified using an epitope specific to GRIP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 122 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons. -
Sequence similarities
Contains 7 PDZ (DHR) domains. -
Domain
PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR. -
Cellular localization
Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 452059 Chimpanzee
- Entrez Gene: 109029244 Gorilla
- Entrez Gene: 23426 Human
- Entrez Gene: 100442221 Orangutan
- Entrez Gene: 717885 Rhesus monkey
- Omim: 604597 Human
- SwissProt: Q9Y3R0 Human
- Unigene: 505946 Human
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Alternative names
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
see all
Images
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Western blot - Anti-GRIP1 antibody (ab168848)All lanes : Anti-GRIP1 antibody (ab168848) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-GRIP1 antibody (ab168848)Detection of GRIP1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168848 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References
ab168848 has not yet been referenced specifically in any publications.
