Key features and details
- Rabbit polyclonal to GRIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRIP1 antibody
See all GRIP1 primary antibodies
DescriptionRabbit polyclonal to GRIP1
SpecificityThis antibody is raised against glutamate receptor-interacting protein 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
- 293T whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168848 was affinity purified using an epitope specific to GRIP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab168848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
Sequence similaritiesContains 7 PDZ (DHR) domains.
DomainPDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
Cellular localizationCytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
- Information by UniProt
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
All lanes : Anti-GRIP1 antibody (ab168848) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 minutes
Detection of GRIP1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168848 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab168848 has not yet been referenced specifically in any publications.