Key features and details
- Rabbit polyclonal to GRIP1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GRIP1 antibody
See all GRIP1 primary antibodies
DescriptionRabbit polyclonal to GRIP1
SpecificityThis antibody is raised against glutamate receptor-interacting protein 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- This antibody gave a positive signal in Mouse Brain Tissue Lysate - postnatal day 7, Rat Brain Tissue Lysate - normal adult tissue, Rat Brain Tissue Lysate - postnatal day 7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab25963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).
Abcam recommends using BSA as the blocking agent.
FunctionMay play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
Sequence similaritiesContains 7 PDZ (DHR) domains.
DomainPDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
Cellular localizationCytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
- Information by UniProt
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab25963 has been referenced in 2 publications.
- Barnes SJ et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron 96:871-882.e5 (2017). IHC-P . PubMed: 29107520
- Gainey MA et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A 112:E3590-9 (2015). PubMed: 26109571