• Product name

  • Description

    Rabbit polyclonal to GRIP1
  • Host species

  • Specificity

    This antibody is raised against glutamate receptor-interacting protein 1
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse GRIP1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab27748.)

  • Positive control

    • This antibody gave a positive signal in Mouse Brain Tissue Lysate - postnatal day 7, Rat Brain Tissue Lysate - normal adult tissue, Rat Brain Tissue Lysate - postnatal day 7.



Our Abpromise guarantee covers the use of ab25963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).

Abcam recommends using BSA as the blocking agent.


  • Function

    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
  • Sequence similarities

    Contains 7 PDZ (DHR) domains.
  • Domain

    PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
  • Cellular localization

    Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • Database links

  • Alternative names

    • as GRIP antibody
    • Glutamate receptor-interacting protein 1 antibody
    • GRIP-1 antibody
    • Grip1 antibody
    • GRIP1_HUMAN antibody
    see all


  • All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
    Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
    Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7

    Lysates/proteins at 15 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 135 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ab25963 staining GRIP1 (red) in mouse skeletal (gastrocnemius) muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + Tween-20) for 12 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. DAPI (blue) - Nuclei.

    See Abreview


This product has been referenced in:

  • Barnes SJ  et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron 96:871-882.e5 (2017). IHC-P . Read more (PubMed: 29107520) »
  • Gainey MA  et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A 112:E3590-9 (2015). Read more (PubMed: 26109571) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Mouse Cell (skeletal muscle)
skeletal muscle

Abcam user community

Verified customer

Submitted Sep 12 2014

Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain sections)
Brain sections

Dr. Sophie Pezet

Verified customer

Submitted Aug 03 2010

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