The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).
Abcam recommends using BSA as the blocking agent.
May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
Contains 7 PDZ (DHR) domains.
PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Immunocytochemistry/ Immunofluorescence - Anti-GRIP1 antibody (ab25963)This image is courtesy of an anonymous Abreview
ab25963 staining GRIP1 (red) in mouse skeletal (gastrocnemius) muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + Tween-20) for 12 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. DAPI (blue) - Nuclei.
Barnes SJ et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron96:871-882.e5 (2017).
Read more (PubMed: 29107520) »
Gainey MA et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A112:E3590-9 (2015).
Read more (PubMed: 26109571) »