Product nameAnti-GRIP1 antibody
See all GRIP1 primary antibodies
DescriptionRabbit polyclonal to GRIP1
SpecificityThis antibody is raised against glutamate receptor-interacting protein 1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- This antibody gave a positive signal in Mouse Brain Tissue Lysate - postnatal day 7, Rat Brain Tissue Lysate - normal adult tissue, Rat Brain Tissue Lysate - postnatal day 7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab25963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 135 kDa (predicted molecular weight: 122 kDa).
Abcam recommends using BSA as the blocking agent.
FunctionMay play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.
Sequence similaritiesContains 7 PDZ (DHR) domains.
DomainPDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.
Cellular localizationCytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
- Information by UniProt
- as GRIP antibody
- Glutamate receptor-interacting protein 1 antibody
- GRIP-1 antibody
All lanes : Anti-GRIP1 antibody (ab25963) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - postnatal day 7
Lane 2 : Brain (Rat) Tissue Lysate - normal adult tissue
Lane 3 : Brain (Rat) Tissue Lysate - postnatal day 7
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa, 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25963 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab25963 staining GRIP1 (red) in mouse skeletal (gastrocnemius) muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + Tween-20) for 12 hours at 4°C. A Cy3®-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. DAPI (blue) - Nuclei.
This product has been referenced in:
- Barnes SJ et al. Deprivation-Induced Homeostatic Spine Scaling In Vivo Is Localized to Dendritic Branches that Have Undergone Recent Spine Loss. Neuron 96:871-882.e5 (2017). IHC-P . Read more (PubMed: 29107520) »
- Gainey MA et al. Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade. Proc Natl Acad Sci U S A 112:E3590-9 (2015). Read more (PubMed: 26109571) »