Anti-GRIP1 antibody [GRP01] (ab44970)

Reviews (2)Q&A (1)

Key features and details

  • Mouse monoclonal [GRP01] to GRIP1
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-GRIP1 antibody [GRP01]
    See all GRIP1 primary antibodies

  • Description

    Mouse monoclonal [GRP01] to GRIP1

  • Host species

    Mouse

  • Specificity

    This antibody is raised against glutamate receptor-interacting protein 1

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant GRIP1 protein (Human)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Target

  • Function

    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.

  • Sequence similarities

    Contains 7 PDZ (DHR) domains.

  • Domain

    PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.

  • Cellular localization

    Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt

  • Database links

    • Alternative names

      • as GRIP antibody
      • Glutamate receptor-interacting protein 1 antibody
      • GRIP-1 antibody
      • Grip1 antibody
      • GRIP1_HUMAN antibody
      see all

    References (0)

    Publishing research using ab44970? Please let us know so that we can cite the reference in this datasheet.

    ab44970 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    ReviewID: xxx AbID: 44970, Anti-GRIP1 antibody [GRP01] Rating: Poor Image: Anti-GRIP1 antibody [GRP01] for Western blot (Human) Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 20 µg Specification: Stably transfected CHO cells expressing human GRIP Application: Application: Gel Running Conditions: Reduced Denaturing Blocking step: Milk as blocking agent for 1 hour at 20°C Blocking Concentration: 5% Other detail: Dilution: 1/500 Incubation time: 2 hours at 20°C Diluent: PBST Secondary Antibody: Name: Non-Abcam Antibody was used: goat anti mouse Conjugation: HRP Dilution: 1/10000 Detection: Detection method: ECL Exposure: 10 minutes and 0 seconds Positive Control: CHO cells were stably transfected with full length human GRIP1 N-terminally tagged with myc. Cell lysates were analyzed by Western blot. To verify signals: second half of the blot was incubated with non-abcam monoclonal anti-myc antibody Additional Data: Additional Notes: CHO cells were stably transfected with full length human GRIP1 N-terminally tagged with myc. Cell lysates were analyzed by Western blot. To verify signals: second half of the blot was incubated with non-abcam monoclonal anti-myc antibody

    Read More
    Answer

    Thank you for submitting an Abreview of ab44970. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

    - Loading more protein might be of help to obtain the band.
    - Incubation with the primary antibody overnight at 4 degree can help as well.

    If you decide to follow these suggestions, please let me know if they are helpful.

    Additionally, this antibody is covered by our Abpromise, thus if the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

    I look forward to your reply.

    Read More

    Western blot abreview for Anti-GRIP1 antibody [GRP01]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Stably transfected CHO cells expressing human GRIP)
    Loading amount
    20 µg
    Specification
    Stably transfected CHO cells expressing human GRIP
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 28 2012

    Immunocytochemistry abreview for Anti-GRIP1 antibody [GRP01]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (CHO Cells stably transfected with human Grip1)
    Specification
    CHO Cells stably transfected with human Grip1
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2011

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