Anti-GRIP1 antibody [GRP01] (ab44970)

Reviews (2)Q&A (1)

Overview

  • Product name

    Anti-GRIP1 antibody [GRP01]
    See all GRIP1 primary antibodies

  • Description

    Mouse monoclonal [GRP01] to GRIP1

  • Host species

    Mouse

  • Specificity

    This antibody is raised against glutamate receptor-interacting protein 1

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant GRIP1 protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab44970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP 1/400.
WB 1/200. Detects a band of approximately 160 kDa.

Target

  • Function

    May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons.

  • Sequence similarities

    Contains 7 PDZ (DHR) domains.

  • Domain

    PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1 and EPHB2 and with the C-terminus of PPFIA1 and PPFIA4. PDZ 4 and PDZ 5 mediate interaction with the C-terminus of GRIA2 and GRIA3. PDZ 4, PDZ 5 and PDZ 6 mediate homomultimers. PDZ 7 mediates interaction with PDZ domain of GRASP1. PDZ 7 domain binds CSPG4. PDZ 6 mediates interaction with the C-terminus of liprins-alpha. PDZ 1, PDZ 2 and PDZ 3 mediate interaction with the PDZ-binding motif of FRAS1 (By similarity). PDZ 4 and PDZ 5 mediate interaction with PRLHR.

  • Cellular localization

    Cytoplasmic vesicle. Endoplasmic reticulum. Cell junction > synapse > postsynaptic cell membrane. Cytoplasmic and membrane-associated with vesicles, peri-Golgi complexes and endoplasmic reticulum. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt

  • Database links

    • Alternative names

      • as GRIP antibody
      • Glutamate receptor-interacting protein 1 antibody
      • GRIP-1 antibody
      • Grip1 antibody
      • GRIP1_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GRIP1 antibody [GRP01] (ab44970)
      Immunocytochemistry/ Immunofluorescence - Anti-GRIP1 antibody [GRP01] (ab44970)This image is courtesy of an Anonymous Abreview.
      ab44970 staining GRIP1 in CHO Cells stably transfected with human GRIP1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/500 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400.

      See Abreview

    References

    ab44970 has not yet been referenced specifically in any publications.

    Publishing research using ab44970? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    ReviewID: xxx AbID: 44970, Anti-GRIP1 antibody [GRP01] Rating: Poor Image: Anti-GRIP1 antibody [GRP01] for Western blot (Human) Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 20 µg Specification: Stably transfected CHO cells expressing human GRIP Application: Application: Gel Running Conditions: Reduced Denaturing Blocking step: Milk as blocking agent for 1 hour at 20°C Blocking Concentration: 5% Other detail: Dilution: 1/500 Incubation time: 2 hours at 20°C Diluent: PBST Secondary Antibody: Name: Non-Abcam Antibody was used: goat anti mouse Conjugation: HRP Dilution: 1/10000 Detection: Detection method: ECL Exposure: 10 minutes and 0 seconds Positive Control: CHO cells were stably transfected with full length human GRIP1 N-terminally tagged with myc. Cell lysates were analyzed by Western blot. To verify signals: second half of the blot was incubated with non-abcam monoclonal anti-myc antibody Additional Data: Additional Notes: CHO cells were stably transfected with full length human GRIP1 N-terminally tagged with myc. Cell lysates were analyzed by Western blot. To verify signals: second half of the blot was incubated with non-abcam monoclonal anti-myc antibody

    Read More
    Answer

    Thank you for submitting an Abreview of ab44970. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

    - Loading more protein might be of help to obtain the band.
    - Incubation with the primary antibody overnight at 4 degree can help as well.

    If you decide to follow these suggestions, please let me know if they are helpful.

    Additionally, this antibody is covered by our Abpromise, thus if the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

    I look forward to your reply.

    Read More

    Western blot abreview for Anti-GRIP1 antibody [GRP01]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Stably transfected CHO cells expressing human GRIP)
    Loading amount
    20 µg
    Specification
    Stably transfected CHO cells expressing human GRIP
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 28 2012

    Immunocytochemistry abreview for Anti-GRIP1 antibody [GRP01]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (CHO Cells stably transfected with human Grip1)
    Specification
    CHO Cells stably transfected with human Grip1
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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