Anti-GRK1 antibody [D11] (ab2776)
![Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)](https://www.abcam.com/ps/products/2/ab2776/Images/ab2776-219024-ab2776iccif4.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)")
Key features and details
- Mouse monoclonal [D11] to GRK1
- Suitable for: ICC/IF
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-GRK1 antibody [D11]
See all GRK1 primary antibodies -
Description
Mouse monoclonal [D11] to GRK1 -
Host species
Mouse -
Specificity
Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Cow, Human -
Immunogen
Full length native protein (purified) corresponding to Human GRK1.
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Epitope
This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events. -
Clonality
Monoclonal -
Clone number
D11 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/20 - 1/200.
|
| Notes |
|---|
|
ICC/IF
1/20 - 1/200. |
Target
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Function
Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. -
Tissue specificity
Retinal-specific. Expressed in rods and cones cells. -
Involvement in disease
Night blindness, congenital stationary, Oguchi type 2 -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. -
Post-translational
modificationsAutophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 131890 Human
- Entrez Gene: 6011 Human
- Entrez Gene: 24013 Mouse
- Omim: 180381 Human
- SwissProt: Q15835 Human
- SwissProt: Q8WTQ7 Human
- SwissProt: Q9WVL4 Mouse
- Unigene: 103501 Human
see all -
Alternative names
- EC=2.7.11.14 antibody
- G protein-coupled receptor kinase 1 antibody
- GPRK1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)](https://www.abcam.com/ps/products/2/ab2776/Images/ab2776-219022-ab2776iccif3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)](https://www.abcam.com/ps/products/2/ab2776/Images/ab2776-219020-ab2776iccif2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)](https://www.abcam.com/ps/products/2/ab2776/Images/ab2776-219019-ab2776iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)Immunolocalization of GRK1 in bovine retina using ab2776.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab2776 has been referenced in 5 publications.
- Toulis V et al. The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina. Cell Rep 33:108360 (2020). PubMed: 33176149
- Datta P et al. The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. J Biol Chem 294:19119-19136 (2019). PubMed: 31694913
- Miloudi K et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). PubMed: 27400127
- Sakurai K et al. Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors. Invest Ophthalmol Vis Sci 52:6793-800 (2011). WB . PubMed: 21474765
- Higgins MK et al. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem 281:19426-32 (2006). PubMed: 16675451
