Anti-GRK1 antibody [D11] (ab2776)

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Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

Key features and details

  • Mouse monoclonal [D11] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GRK1 antibody [D11]
    See all GRK1 primary antibodies

  • Description

    Mouse monoclonal [D11] to GRK1

  • Host species

    Mouse

  • Specificity

    Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Cow, Human

  • Immunogen

    Full length native protein (purified) corresponding to Human GRK1.

  • Epitope

    This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab2776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/200.

Target

  • Function

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

  • Tissue specificity

    Retinal-specific. Expressed in rods and cones cells.

  • Involvement in disease

    Night blindness, congenital stationary, Oguchi type 2

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.

  • Post-translational
    modifications

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunolocalization of GRK1 in bovine retina using ab2776.

References (3)

Publishing research using ab2776? Please let us know so that we can cite the reference in this datasheet.

ab2776 has been referenced in 3 publications.

  • Miloudi K  et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). PubMed: 27400127
  • Sakurai K  et al. Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors. Invest Ophthalmol Vis Sci 52:6793-800 (2011). WB . PubMed: 21474765
  • Higgins MK  et al. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem 281:19426-32 (2006). PubMed: 16675451

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