Overview

  • Product name
    Anti-GRK1 antibody [D11]
    See all GRK1 primary antibodies
  • Description
    Mouse monoclonal [D11] to GRK1
  • Host species
    Mouse
  • Specificity
    Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human GRK1.

  • Epitope
    This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

Properties

Applications

Our Abpromise guarantee covers the use of ab2776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/200.
IHC-Fr 1/1000.
WB 1/1000.
IP Use at an assay dependent concentration.

Target

  • Function
    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • Tissue specificity
    Retinal-specific. Expressed in rods and cones cells.
  • Involvement in disease
    Night blindness, congenital stationary, Oguchi type 2
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

    Immunolocalization of GRK1 in bovine retina using ab2776.

References

This product has been referenced in:
  • Miloudi K  et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). Read more (PubMed: 27400127) »
  • Sakurai K  et al. Variation in rhodopsin kinase expression alters the dim flash response shut off and the light adaptation in rod photoreceptors. Invest Ophthalmol Vis Sci 52:6793-800 (2011). WB . Read more (PubMed: 21474765) »

See all 3 Publications for this product

Publishing research using ab2776? Please let us know so that we can cite the reference in this datasheet.

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