Overview

  • Product name

  • Description

    Goat polyclonal to GRK2
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: GRK2 HEK-293T overexpressing lysate; THP-1 lysate. IHC-P: Human high grade lymphoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000. Predicted molecular weight: 80 kDa.

Target

  • Function

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificity

    Expressed in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Images

  • Paraffin-embedded human high grade lymphoma tissue stained for GRK2 using ab235240 at 1/1000 dilution in immunohistochemical analysis. Counterstained with Hematoxylin. Magnification 20x.

    Antigen retrieval: Heat induced epitope retrieval (HIER).

    The Abs developed a specific cytoplasmic staining and partially weak nucelear staining in human high grade lymphoma tissue.

  • All lanes : Anti-GRK2 antibody (ab235240) at 1/1000 dilution

    Lane 2 : GRK2 HEK-293T over expressing lysate.
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) empty vector lysate.

    Secondary
    All lanes : Dk-a-Goat IgG [H&L] HRP at 1/40000 dilution

    Predicted band size: 80 kDa


    Exposure time: 120 seconds


    Blocking/diluting buffer: 5% BLOTTO.

  • Anti-GRK2 antibody (ab235240) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate

    Secondary
    Dk-a-Goat IgG [H&L] HRP at 1/40000 dilution

    Predicted band size: 80 kDa


    Exposure time: 120 seconds


    Blocking/Diluting buffer and concentration: 5% BLOTTO.

References

ab235240 has not yet been referenced specifically in any publications.

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