Anti-GRK2 antibody - C-terminal (ab137666)

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Overview

  • Product name

    Anti-GRK2 antibody - C-terminal
    See all GRK2 primary antibodies

  • Description

    Rabbit polyclonal to GRK2 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow

  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 477-689 of Human GRK2.

  • Positive control

    • 293T, A431, HeLaS3, HepG2, and Raji cell lines. OVCAR3 xenograft; methanol-fixed HeLa; NIH-3T3 and MOLT4 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab137666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.

Target

  • Function

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.

  • Tissue specificity

    Expressed in peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Images

  • Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)
    Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.

References

This product has been referenced in:

  • Hou X  et al. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows. J Dairy Sci 99:5828-5836 (2016). Read more (PubMed: 27132107) »
  • Xia SH  et al. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 19:3969-79 (2013). WB ; Mouse . Read more (PubMed: 23840141) »
See all 2 Publications for this product

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