Anti-GRK2 antibody - C-terminal (ab137666)
Key features and details
- Rabbit polyclonal to GRK2 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GRK2 antibody - C-terminal
See all GRK2 primary antibodies -
Description
Rabbit polyclonal to GRK2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
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Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 477-689 of Human GRK2.
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Positive control
- 293T, A431, HeLaS3, HepG2, and Raji cell lines. OVCAR3 xenograft; methanol-fixed HeLa; NIH-3T3 and MOLT4 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab137666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
Target
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Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
Tissue specificity
Expressed in peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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Database links
- Entrez Gene: 282682 Cow
- Entrez Gene: 156 Human
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P21146 Cow
- SwissProt: P25098 Human
- SwissProt: Q99MK8 Mouse
- SwissProt: P26817 Rat
see all -
Alternative names
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
Images
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Western blot - Anti-GRK2 antibody - C-terminal (ab137666)Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Western blot - Anti-GRK2 antibody - C-terminal (ab137666)Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.
Protocols
References (2)
ab137666 has been referenced in 2 publications.
- Hou X et al. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows. J Dairy Sci 99:5828-5836 (2016). PubMed: 27132107
- Xia SH et al. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 19:3969-79 (2013). WB ; Mouse . PubMed: 23840141
