Anti-GRK2 antibody - C-terminal (ab137666)
Key features and details
- Rabbit polyclonal to GRK2 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GRK2 antibody - C-terminal
See all GRK2 primary antibodies -
Description
Rabbit polyclonal to GRK2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
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Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 477-689 of Human GRK2.
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Positive control
- 293T, A431, HeLaS3, HepG2, and Raji cell lines. OVCAR3 xenograft; methanol-fixed HeLa; NIH-3T3 and MOLT4 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
Target
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Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
Tissue specificity
Expressed in peripheral blood leukocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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Database links
- Entrez Gene: 282682 Cow
- Entrez Gene: 156 Human
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P21146 Cow
- SwissProt: P25098 Human
- SwissProt: Q99MK8 Mouse
- SwissProt: P26817 Rat
see all -
Alternative names
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
Images
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Western blot - Anti-GRK2 antibody - C-terminal (ab137666)Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Western blot - Anti-GRK2 antibody - C-terminal (ab137666)Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137666 has been referenced in 3 publications.
- Hu J et al. LDK378 inhibits the recruitment of myeloid-derived suppressor cells to spleen via the p38-GRK2-CCR2 pathway in mice with sepsis. Immunol Cell Biol 97:902-915 (2019). PubMed: 31472096
- Hou X et al. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows. J Dairy Sci 99:5828-5836 (2016). PubMed: 27132107
- Xia SH et al. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 19:3969-79 (2013). WB ; Mouse . PubMed: 23840141