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  1. Link

    grk2-antibody-c-terminal-ab137666.pdf

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Anti-GRK2 antibody - C-terminal (ab137666)

  • Datasheet
  • SDS
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Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
  • Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)

Key features and details

  • Rabbit polyclonal to GRK2 - C-terminal
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GRK2 antibody [EPR22465] (ab227825)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GRK2 antibody - C-terminal
    See all GRK2 primary antibodies
  • Description

    Rabbit polyclonal to GRK2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 477-689 of Human GRK2.

  • Positive control

    • 293T, A431, HeLaS3, HepG2, and Raji cell lines. OVCAR3 xenograft; methanol-fixed HeLa; NIH-3T3 and MOLT4 whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Adrenergic Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • NIH/3T3 whole cell lysate (ab7179)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human GRK2 protein (ab125620)
  • Related Products

    • Recombinant human GRK2 protein (ab125620)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.

Target

  • Function

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificity

    Expressed in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Target information above from: UniProt accession P25098 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282682 Cow
    • Entrez Gene: 156 Human
    • Entrez Gene: 25238 Rat
    • Omim: 109635 Human
    • SwissProt: P21146 Cow
    • SwissProt: P25098 Human
    • SwissProt: Q99MK8 Mouse
    • SwissProt: P26817 Rat
    • Unigene: 83636 Human
    • Unigene: 254144 Mouse
    • Unigene: 13010 Rat
    see all
  • Alternative names

    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Images

  • Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Western blot - Anti-GRK2 antibody - C-terminal (ab137666)
    Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK2 antibody - C-terminal (ab137666)
    Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK2 antibody - C-terminal (ab137666)
    Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab137666? Please let us know so that we can cite the reference in this datasheet.

ab137666 has been referenced in 3 publications.

  • Hu J  et al. LDK378 inhibits the recruitment of myeloid-derived suppressor cells to spleen via the p38-GRK2-CCR2 pathway in mice with sepsis. Immunol Cell Biol 97:902-915 (2019). PubMed: 31472096
  • Hou X  et al. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows. J Dairy Sci 99:5828-5836 (2016). PubMed: 27132107
  • Xia SH  et al. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 19:3969-79 (2013). WB ; Mouse . PubMed: 23840141

Customer reviews and Q&As

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