Key features and details
- Rabbit polyclonal to GRK2 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GRK2 antibody - C-terminal
See all GRK2 primary antibodies
DescriptionRabbit polyclonal to GRK2 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
Synthetic peptide, corresponding to a region within C terminal amino acids 477-689 of Human GRK2.
- 293T, A431, HeLaS3, HepG2, and Raji cell lines. OVCAR3 xenograft; methanol-fixed HeLa; NIH-3T3 and MOLT4 whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 80 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.|
FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Tissue specificityExpressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
- Information by UniProt
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE
Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE
Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labelling GRK2 (Green) with ab137666 at 1/100 dilution. Alpha-tubulin filaments were also labelled (Red).
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft labelling GRK2 with ab137666 at 1/500 dilution.
ab137666 has been referenced in 2 publications.
- Hou X et al. The effect of G protein-coupled receptor kinase 2 (GRK2) on lactation and on proliferation of mammary epithelial cells from dairy cows. J Dairy Sci 99:5828-5836 (2016). PubMed: 27132107
- Xia SH et al. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 19:3969-79 (2013). WB ; Mouse . PubMed: 23840141