Product nameAnti-GRK2 antibody - C-terminal
See all GRK2 primary antibodies
DescriptionRabbit polyclonal to GRK2 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide within Human GRK2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: P25098
- WB: Jurkat, Raji and NCI-H929 whole cell lysate. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 80 kDa.|
|IP||1/100 - 1/500.|
FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Tissue specificityExpressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
- Information by UniProt
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
GRK2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts with 5 μg ab229669. Western blot was then performed from the immunoprecipitate using ab229669. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: Jurkat whole cell extract (input)
Lane 2: Control IgG instead of ab229669 in Jurkat whole cell extract.
Lane 3: ab229669 IP in Jurkat whole cell extract.
All lanes : Anti-GRK2 antibody - C-terminal (ab229669) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS-PAGE gel.
ab229669 has not yet been referenced specifically in any publications.