Overview

  • Product name

    Anti-GRK2 antibody - C-terminal
    See all GRK2 primary antibodies
  • Description

    Rabbit polyclonal to GRK2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide within Human GRK2 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated
    Database link: P25098

  • Positive control

    • WB: Jurkat, Raji and NCI-H929 whole cell lysate. IP: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab229669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Tissue specificity

    Expressed in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADRBK1 antibody
    • Adrenergic beta receptor kinase 1 antibody
    • ARBK1_HUMAN antibody
    • BARK antibody
    • BARK1 antibody
    • Beta adrenergic receptor kinase 1 antibody
    • Beta ARK 1 antibody
    • Beta ARK1 antibody
    • Beta-adrenergic receptor kinase 1 antibody
    • Beta-ARK-1 antibody
    • FLJ16718 antibody
    • G protein coupled receptor kinase 2 antibody
    • G-protein coupled receptor kinase 2 antibody
    • GRK2 antibody
    see all

Images

  • GRK2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts with 5 μg ab229669. Western blot was then performed from the immunoprecipitate using ab229669. Anti-Rabbit IgG was used as a secondary antibody.

    Lane 1: Jurkat whole cell extract (input)
    Lane 2: Control IgG instead of ab229669 in Jurkat whole cell extract.
    Lane 3: ab229669 IP in Jurkat whole cell extract.

  • All lanes : Anti-GRK2 antibody - C-terminal (ab229669) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS-PAGE gel.

References

ab229669 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Pig Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-12% bis tris)
Loading amount
50 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jul 16 2018

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