Key features and details
- Rabbit polyclonal to GRK2 (phospho S29)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRK2 (phospho S29) antibody
See all GRK2 primary antibodies
DescriptionRabbit polyclonal to GRK2 (phospho S29)
SpecificityThis antibody detects endogenous levels of GRK2 only when phosphorylated at serine 29.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthesized phosphopeptide derived from human GRK2 around the phosphorylation site of serine 29 (R-A-SP-K-K).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab58520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 80 kDa.|
FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Tissue specificityExpressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
- Information by UniProt
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)
Lane 1 : Jurkat cells treated with EGF
Lane 2 : Jurkat cells treated with EGF with phospho peptide
Predicted band size: 80 kDa
This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
ab58520 has been referenced in 1 publication.
- Schreckenberg R et al. Adverse Effects on ß-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function. J Am Heart Assoc 6:N/A (2017). PubMed: 29273639