Anti-GRK2 (phospho S685) antibody (ab138438)

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Western blot - Anti-GRK2 (phospho S685) antibody (ab138438)

    Key features and details

    • Rabbit polyclonal to GRK2 (phospho S685)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-GRK2 (phospho S685) antibody
      See all GRK2 primary antibodies

    • Description

      Rabbit polyclonal to GRK2 (phospho S685)

    • Host species

      Rabbit

    • Specificity

      ab138438 detects endogenous levels of GRK2 only when phosphorylated at S685.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat

    • Immunogen

      Synthetic phospho-peptide against the C terminal region of Human GRK2, around S685 (UniProt ID: P25098).

    • Positive control

      • Extracts from HT29 cells treated with 0.01 U/ml of insulin for 15 minutes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20ºC.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab138438 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab138438 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.

    Target

    • Function

      Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.

    • Tissue specificity

      Expressed in peripheral blood leukocytes.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 RGS domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ADRBK1 antibody
      • Adrenergic beta receptor kinase 1 antibody
      • ARBK1_HUMAN antibody
      • BARK antibody
      • BARK1 antibody
      • Beta adrenergic receptor kinase 1 antibody
      • Beta ARK 1 antibody
      • Beta ARK1 antibody
      • Beta-adrenergic receptor kinase 1 antibody
      • Beta-ARK-1 antibody
      • FLJ16718 antibody
      • G protein coupled receptor kinase 2 antibody
      • G-protein coupled receptor kinase 2 antibody
      • GRK2 antibody
      see all

    Images

    • Western blot - Anti-GRK2 (phospho S685) antibody (ab138438)
      Western blot - Anti-GRK2 (phospho S685) antibody (ab138438)
      Anti-GRK2 (phospho S685) antibody (ab138438) at 1/500 dilution + HT29 cells treated with 0.01 U/ml insulin for 15 min

      Predicted band size: 80 kDa

    References (0)

    Publishing research using ab138438? Please let us know so that we can cite the reference in this datasheet.

    ab138438 has not yet been referenced specifically in any publications.

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