Key features and details
- Rabbit polyclonal to GRK3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG
Product nameAnti-GRK3 antibody
See all GRK3 primary antibodies
DescriptionRabbit polyclonal to GRK3
SpecificityTargets C terminal of GRK3. The antibody might crossreact with GRK2 (immunogen sequence is 100% homolog with GRK2), but we do not have any testing data showing cross reactivity with GRK2.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Monkey
Synthetic peptide conjugated to KLH and selected from the C terminal region of GRK3 (Human)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS
Our Abpromise guarantee covers the use of ab38294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSpecifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
- Information by UniProt
- ADRBK2 antibody
- Adrenergic, beta, receptor kinase 2 antibody
- ARBK2_HUMAN antibody
Anti-GRK3 antibody (ab38294) + Mouse heart tissue lysate
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
IHC image of ab38294 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38294, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab38294 has been referenced in 2 publications.
- Jiang T et al. Overexpression of GRK3, Promoting Tumor Proliferation, Is Predictive of Poor Prognosis in Colon Cancer. Dis Markers 2017:1202710 (2017). PubMed: 29445249
- McCormick PJ et al. Impaired recruitment of Grk6 and beta-Arrestin 2 causes delayed internalization and desensitization of a WHIM syndrome-associated CXCR4 mutant receptor. PLoS One 4:e8102 (2009). WB, ICC/IF ; Human . PubMed: 19956569