Anti-GRK3 antibody - N-terminal (ab137764)
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Overview
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Product name
Anti-GRK3 antibody - N-terminal
See all GRK3 primary antibodies -
Description
Rabbit polyclonal to GRK3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-225 of Human GRK3 (Uniprot ID: P35626).
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Positive control
- HeLa whole cell lysate, Human H1299 xenograft tissue, and MOLT4 cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab137764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
Target
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Function
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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Database links
- Entrez Gene: 282136 Cow
- Entrez Gene: 157 Human
- Entrez Gene: 320129 Mouse
- Entrez Gene: 25372 Rat
- Omim: 109636 Human
- SwissProt: P26818 Cow
- SwissProt: P35626 Human
- SwissProt: Q3UYH7 Mouse
see all -
Alternative names
- ADRBK2 antibody
- Adrenergic, beta, receptor kinase 2 antibody
- ARBK2_HUMAN antibody
see all
Images
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Western blot - Anti-GRK3 antibody - N-terminal (ab137764)Anti-GRK3 antibody - N-terminal (ab137764) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody - N-terminal (ab137764)Immunohistochemical analysis of paraffin-embedded Human HT1299 xenograft tissue, labelling GRK3 with ab137764 at 1/100 dilution.
References
ab137764 has not yet been referenced specifically in any publications.
