Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 67 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionSpecifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
Tissue specificityIsoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
Cellular localizationCytoplasm. Cytoplasm > cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where co-localization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
- Information by UniProt
- G protein coupled receptor kinase 4 antibody
- G protein-coupled receptor kinase 2 (Drosophila)-like antibody
- G protein-coupled receptor kinase 4 antibody
All lanes : Anti-GRK4 antibody (ab182635) at 1/700 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 40 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Predicted band size: 67 kDa
Exposure time: 40 seconds
Gel: 10% SDS-PAGE
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling GRK4 with ab182635 at 1/40 dilution (left panel). Right panel treated with the immunogen.
ab182635 has not yet been referenced specifically in any publications.