Overview

  • Product name

  • Description

    Rabbit polyclonal to GRK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GRK4 aa 1-245.
    Database link: P32298

  • Positive control

    • WB: Jurkat whole cell lysate. IHC-P: Human testis and liver cancer tissues. ICC/IF: PC-3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 66 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.
  • Tissue specificity

    Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • Post-translational
    modifications

    Palmitoylated.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where co-localization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor kinase 4 antibody
    • G protein-coupled receptor kinase 2 (Drosophila)-like antibody
    • G protein-coupled receptor kinase 4 antibody
    • G protein-coupled receptor kinase GRK4 antibody
    • G proteinm coupled receptor kinase GRK4 antibody
    • GPRK2L antibody
    • GPRK4 antibody
    • GRK4 antibody
    • GRK4_HUMAN antibody
    • GRK4a antibody
    • ITI1 antibody
    see all

Images

  • Anti-GRK4 antibody (ab236645) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Secondary
    Goat polyclonal to rabbit at 1/10000 dilution

    Predicted band size: 66 kDa

  • Paraffin-embedded human testis tissue stained for GRK4 using ab236645 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver cancer tissue stained for GRK4 using ab236645 at 1/100 dilution in immunohistochemical analysis.

  • PC-3 (human prostate adenocarcinoma cell line) cells stained for GRK4 (green) using ab236645 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236645 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab236645.
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