Product nameAnti-GRK5 antibody
See all GRK5 primary antibodies
DescriptionRabbit polyclonal to GRK5
SpecificityGRK5 antibody detects endogenous levels of total GRK5 protein.
Tested applicationsSuitable for: IHC-Fr, WB, ELISA, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide from internal of human GRK5.
- HUVEC, HepG2 and HeLa cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSpecifically phosphorylates the activated forms of G protein-coupled receptors.
Tissue specificityHighest levels in heart, placenta, lung > skeletal muscle > brain, liver, pancreas > kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
- Information by UniProt
- FLJ39780 antibody
- FP2025 antibody
- G protein coupled receptor kinase 5 antibody
All lanes : Anti-GRK5 antibody (ab64943) at 1/500 dilution
Lane 1 : HUVEC extract
Lane 2 : HepG2 cell extract
Lane 3 : HeLa cell extract
Lane 4 : HeLa cell extract with immunising peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 100 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
ICC/IF image of ab64943 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab64943 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64943, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Funk AJ et al. Increased G protein-coupled receptor kinase (GRK) expression in the anterior cingulate cortex in schizophrenia. Schizophr Res 159:130-5 (2014). Read more (PubMed: 25153362) »
- Rotermund C et al. Diet-induced obesity accelerates the onset of terminal phenotypes in a-synuclein transgenic mice. J Neurochem 131:848-58 (2014). Read more (PubMed: 24995537) »