Key features and details
- Mouse monoclonal [2389D3a] to GRLF1
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab58674 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab58674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa for the recombinant fragment (predicted molecular weight for the full length protein: 172 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepresses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Sequence similaritiesContains 4 FF domains.
Contains 1 Rho-GAP domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ARHGAP35 antibody
- GAP associated protein p190 antibody
- Glucocorticoid receptor DNA binding factor 1 antibody
Anti-GRLF1 antibody [2389D3a] (ab58674) + immunising recombinant protein
Predicted band size: 172 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab58674 has not yet been referenced specifically in any publications.