Overview

  • Product name

    Anti-GRLF1 antibody [2389D3a]
    See all GRLF1 primary antibodies
  • Description

    Mouse monoclonal [2389D3a] to GRLF1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human GRLF1

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab58674 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    2389D3a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 35 kDa for the recombinant fragment (predicted molecular weight for the full length protein: 172 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
    • Sequence similarities

      Contains 4 FF domains.
      Contains 1 Rho-GAP domain.
    • Post-translational
      modifications

      Tyrosine phosphorylated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARHGAP35 antibody
      • GAP associated protein p190 antibody
      • Glucocorticoid receptor DNA binding factor 1 antibody
      • Glucocorticoid receptor DNA-binding factor 1 antibody
      • Glucocorticoid receptor repression factor 1 antibody
      • GRF 1 antibody
      • GRF-1 antibody
      • GRF1 antibody
      • GRLF 1 antibody
      • GRLF1 antibody
      • GRLF1_HUMAN antibody
      • KIAA1722 antibody
      • MGC10745 antibody
      • p190 A antibody
      • p190-A antibody
      • P190A antibody
      • P190A, rat, homolog of antibody
      • p190ARhoGAP antibody
      • p190RhoGAP antibody
      • Rho GAP p190A antibody
      • Rho GTPase-activating protein 35 antibody
      see all

    Images

    References

    ab58674 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab58674.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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