Key features and details
- Rabbit polyclonal to GRLF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GRLF1 antibody
See all GRLF1 primary antibodies
DescriptionRabbit polyclonal to GRLF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 1200 and 1250 of human GRLF1 (EAW57452.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85950 was affinity purified using an epitope specific to GRLF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 172 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRepresses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Sequence similaritiesContains 4 FF domains.
Contains 1 Rho-GAP domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- ARHGAP35 antibody
- GAP associated protein p190 antibody
- Glucocorticoid receptor DNA binding factor 1 antibody
All lanes : Anti-GRLF1 antibody (ab85950) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 172 kDa
Observed band size: 172 kDa
Exposure time: 10 seconds
Detection of human GRLF1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85950 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab85950 has not yet been referenced specifically in any publications.