Overview

  • Product name

    Anti-GRLF1 (phospho Y1105) antibody
    See all GRLF1 primary antibodies
  • Description

    Rabbit polyclonal to GRLF1 (phospho Y1105)
  • Host species

    Rabbit
  • Specificity

    ab182636 detects endogenous levels of GRLF1 only when phosphorylated at Tyrosine 1150.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GRLF1 (phospho Y1105) conjugated to Keyhole Limpet Haemocyanin (KLH). (N-I-Y(p)-S-V).
    Database link: Q9NRY4

  • Positive control

    • Mouse brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab182636 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 171 kDa.

Target

  • Function

    Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
  • Sequence similarities

    Contains 4 FF domains.
    Contains 1 Rho-GAP domain.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGAP35 antibody
    • GAP associated protein p190 antibody
    • Glucocorticoid receptor DNA binding factor 1 antibody
    • Glucocorticoid receptor DNA-binding factor 1 antibody
    • Glucocorticoid receptor repression factor 1 antibody
    • GRF 1 antibody
    • GRF-1 antibody
    • GRF1 antibody
    • GRLF 1 antibody
    • GRLF1 antibody
    • GRLF1_HUMAN antibody
    • KIAA1722 antibody
    • MGC10745 antibody
    • p190 A antibody
    • p190-A antibody
    • P190A antibody
    • P190A, rat, homolog of antibody
    • p190ARhoGAP antibody
    • p190RhoGAP antibody
    • Rho GAP p190A antibody
    • Rho GTPase-activating protein 35 antibody
    see all

Images

  • All lanes : Anti-GRLF1 (phospho Y1105) antibody (ab182636) at 1/500 dilution

    Lane 1 : Mouse brain tissue lysate with immunizing peptide
    Lane 2 : Mouse brain tissue lysate

    Predicted band size: 171 kDa

References

This product has been referenced in:

  • Yu L  et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). Read more (PubMed: 28791377) »
See 1 Publication for this product

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