Recombinant Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
See all GRO alpha+GRO gamma+GRO beta primary antibodies -
Description
Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
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Positive control
- Human GRO alpha recombinant protein.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4902 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab124898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). |
Target
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Cellular localization
GRO alpha: Secreted. GRO gamma: Secreted. -
Database links
- Entrez Gene: 2919 Human
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P19875 Human
- SwissProt: P19876 Human
- Unigene: 708652 Human
see all
Images
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All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,36,10 kDa why is the actual band size different from the predicted?Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
Datasheets and documents
References (1)
ab124898 has been referenced in 1 publication.
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). PubMed: 29180664