Recombinant Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
See all GRO alpha+GRO gamma+GRO beta primary antibodies -
Description
Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
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Positive control
- Human GRO alpha recombinant protein.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4902 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab124898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). |
Target
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Cellular localization
GRO alpha: Secreted. GRO gamma: Secreted. -
Database links
- Entrez Gene: 2919 Human
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P19875 Human
- SwissProt: P19876 Human
- Unigene: 708652 Human
see all
Images
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![Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)](https://www.abcam.com/ps/products/124/ab124898/Images/ab124898-261062-anti-gro-alpha-gro-gamma-gro-beta-antibody-epr4902-western-blot.jpg)
Western blot - Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,36,10 kDa why is the actual band size different from the predicted?Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). Read more (PubMed: 29180664) »
