Overview

  • Product name

  • Description

    Rabbit polyclonal to GRO alpha
  • Host species

    Rabbit
  • Specificity

    The immunogen for ab86436 has >90% homology to mouse GRO beta
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding amino acid 78 of mouse GRO alpha.

  • Positive control

    • Jurkat and 3T3 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.5% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).

Target

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 1 antibody
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
    • chemokine (C-X-C motif) ligand 1 antibody
    • CINC-1 antibody
    • CXCL1 antibody
    • Cytokine-induced neutrophil chemoattractant 1 antibody
    • Fibroblast secretory protein antibody
    • Fsp antibody
    • Gro 1 antibody
    • Gro A antibody
    • Gro antibody
    • GRO protein, alpha antibody
    • GRO-alpha(1-73) antibody
    • GRO-alpha(6-73) antibody
    • Gro1 antibody
    • GRO1 oncogene (melanoma growth stimulating activity, alpha) antibody
    • GRO1 oncogene (melanoma growth-stimulating activity) antibody
    • Gro1 oncogene antibody
    • GROa antibody
    • GROA_HUMAN antibody
    • Growth-regulated alpha protein antibody
    • KC antibody
    • KC chemokine, mouse, homolog of antibody
    • melanoma growth stimulatory activity alpha antibody
    • Melanoma growth stimulatory activity antibody
    • Melanoma growth stimulatory activity, alpha antibody
    • MGSA alpha antibody
    • MGSA antibody
    • MGSA-a antibody
    • N51 antibody
    • NAP-3 antibody
    • NAP3 antibody
    • Neutrophil-activating protein 3 antibody
    • Platelet-derived growth factor-inducible protein KC antibody
    • Scyb 1 antibody
    • Scyb1 antibody
    • Secretory protein N51 antibody
    • Small inducible cytokine subfamily B, member 1 antibody
    see all

Images

  • ICC/IF image of ab86436 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86436, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GRO alpha antibody (ab86436) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : 3T3 cell lysate

    Predicted band size: 11 kDa
    Observed band size: 9 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zhou J  et al. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment. J Leukoc Biol 105:177-186 (2019). Read more (PubMed: 30351498) »
  • Niu XL  et al. Interleukin-18 exacerbates skin inflammation and affects microabscesses and scale formation in a mouse model of imiquimod-induced psoriasis. Chin Med J (Engl) 132:690-698 (2019). Read more (PubMed: 30741833) »
See all 15 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Tissue sections (Intervertebral Discs))
Specification
Human Tissue sections (Intervertebral Discs)
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris Buffer pH:9,5 for 2x5’ in MW 40% power
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 11 2012

Question
Answer

Thank you for contacting us today.

I am very pleased to hear you would like to test ab86436, ab9841and ab10064with IHC ofparaffin embedded human tissue sections. The codes below will each give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews


ab86436
DISCOUNT CODE: xxxxx

Expiration date: 2012-06-04


ab9841
DISCOUNT CODE: xxxxx

Expiration date: 2012-06-04

ab10064

DISCOUNT CODE: xxxxxx

Expiration date: 2012-06-04

As discussed over the phone, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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