Product nameAnti-GRO alpha antibody (Biotin)
See all GRO alpha primary antibodies
DescriptionRabbit polyclonal to GRO alpha (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Predicted to work with: Mouse, Rat
- WB: Recombinant mouse GRO alpha protein.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml.
Mouse Growth-regulated alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271204. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGROA protein.
FunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
To detect mouse GRO alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant mouse GRO alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse GRO alpha, respectively.
Mouse GRO alpha was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271204. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mouse GRO alpha protein.
KC = GRO alpha
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271204 has not yet been referenced specifically in any publications.