Product nameAnti-GRO alpha antibody (Biotin)
See all GRO alpha primary antibodies
DescriptionRabbit polyclonal to GRO alpha (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- WB: Recombinant human GRO alpha.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human GRO alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- C-X-C motif chemokine 1 antibody
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
- chemokine (C-X-C motif) ligand 1 antibody
Lane 1: Marker.
Lane 2: Recombinant hGRO alpha at 250ng/lane.
Lane 3: Recombinant hGRO alpha at 125ng/lane.
Lane 4: Recombinant hGRO alpha at 62.5ng/lane.
Lane 5: Recombinant hGRO alpha at 31.25ng/lane.
Lane 6: Recombinant hGRO alpha at 15.625ng/lane.
Lane 7: Recombinant hGRO alpha at 7.8ng/lane.
Lane 8: Recombinant hGRO alpha at 3.9ng/lane.
Lane 9: Recombinant hGRO alpha at 1.95ng/lane.
Lane 10: Recombinant hGRO alpha at 0.975ng/lane.
Lane 11: Recombinant hGRO alpha at 0.4875ng/lane.
Lane 12: Recombinant hGRO alpha at 0.24ng/lane.
Human GRO alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271282. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant GRO alpha protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271282 has not yet been referenced specifically in any publications.