Overview

  • Product name

    Anti-GRO alpha antibody [EPR19892]
    See all GRO alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR19892] to GRO alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GRO alpha aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P09341

  • Positive control

    • WB: Human GRO alpha active protein; human GRO beta fragment recombinant protein; human GRO gamma fragment recombinant protein; human fetal lung lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19892
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 1 antibody
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
    • chemokine (C-X-C motif) ligand 1 antibody
    • CINC-1 antibody
    • CXCL1 antibody
    • Cytokine-induced neutrophil chemoattractant 1 antibody
    • Fibroblast secretory protein antibody
    • Fsp antibody
    • Gro 1 antibody
    • Gro A antibody
    • Gro antibody
    • GRO protein, alpha antibody
    • GRO-alpha(1-73) antibody
    • GRO-alpha(6-73) antibody
    • Gro1 antibody
    • GRO1 oncogene (melanoma growth stimulating activity, alpha) antibody
    • GRO1 oncogene (melanoma growth-stimulating activity) antibody
    • Gro1 oncogene antibody
    • GROa antibody
    • GROA_HUMAN antibody
    • Growth-regulated alpha protein antibody
    • KC antibody
    • KC chemokine, mouse, homolog of antibody
    • melanoma growth stimulatory activity alpha antibody
    • Melanoma growth stimulatory activity antibody
    • Melanoma growth stimulatory activity, alpha antibody
    • MGSA alpha antibody
    • MGSA antibody
    • MGSA-a antibody
    • N51 antibody
    • NAP-3 antibody
    • NAP3 antibody
    • Neutrophil-activating protein 3 antibody
    • Platelet-derived growth factor-inducible protein KC antibody
    • Scyb 1 antibody
    • Scyb1 antibody
    • Secretory protein N51 antibody
    • Small inducible cytokine subfamily B, member 1 antibody
    see all

Images

  • All lanes : Anti-GRO alpha antibody [EPR19892] (ab206411) at 1/1000 dilution

    Lane 1 : Human GRO alpha active protein
    Lane 2 : Human GRO beta fragment recombinant protein
    Lane 3 : Human GRO gamma fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 10,36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human GRO beta fragment recombinant protein contain aa35-107 with a GST-Tag. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag®. These two protein were made in house.

  • Anti-GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution + Human fetal lung lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab206411 has not yet been referenced specifically in any publications.

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