Recombinant
RabMAb

Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898)

Overview

  • Product name
    Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
  • Description
    Rabbit monoclonal [EPR4902] to GRO alpha + GRO gamma + GRO beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human GRO alpha recombinant protein.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR4902
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution

      Lane 1 : Human GRO alpha active protein
      Lane 2 : Human GRO beta fragment recombinant protein
      Lane 3 : Human GRO gamma fragment recombinant protein

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
      Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 10,36,10 kDa (why is the actual band size different from the predicted?)



      Exposure time: 3 minutes

      Blocking buffer and concentartion:5% NFDM/TBST

      Diluting buffer and concentration:5% NFDM/TBST

      Human GRO beta fragment pritein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.

    References

    This product has been referenced in:
    • Pasquier J  et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). Read more (PubMed: 29180664) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124898.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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