Overview

  • Product name
  • Description
    Rabbit polyclonal to GRO gamma
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hGROg (human GRO-gamma)

  • Positive control
    • Recombinant human GRO gamma protein (ab9844) can be used as a positive control in WB.

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
To detect hGROg by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGROg is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration.

To detect hGROg by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGROg.

Neutralising Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of hGROg (100.00 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required.

Target

  • Function
    Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 3 antibody
    • C-X-C motif chemokine ligand 3 antibody
    • Chemokine (C X C motif) ligand 3 antibody
    • Chemokine (CXC motif) ligand 3 antibody
    • Cinc 2 antibody
    • CINC 2b antibody
    • Cinc2 antibody
    • CINC2b antibody
    • CXCL 3 antibody
    • Cxcl3 antibody
    • CXCL3_HUMAN antibody
    • Cytokine induced neutrophil chemoattractant 2 antibody
    • Dcip1 antibody
    • Dendritic cell inflammatory protein 1 antibody
    • Gm1960 antibody
    • GRO protein gamma antibody
    • GRO-gamma antibody
    • GRO-gamma(1-73) antibody
    • GRO-gamma(5-73) antibody
    • GRO3 antibody
    • GRO3 oncogene antibody
    • GROG antibody
    • Growth regulated protein gamma antibody
    • Growth-regulated protein gamma antibody
    • Macrophage inflammatory protein 2 beta precursor antibody
    • Macrophage inflammatory protein 2-beta antibody
    • Melanoma growth stimulatory activity gamma antibody
    • Member 3 antibody
    • MGSA gamma antibody
    • MIP 2b antibody
    • MIP2-beta antibody
    • MIP2B antibody
    • SCYB3 antibody
    • Small inducible cytokine subfamily B antibody
    see all

Images

References

ab9843 has not yet been referenced specifically in any publications.

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