Recombinant Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
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Overview
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Product name
Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free
See all GRO gamma primary antibodies -
Description
Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6W5C0 -
Positive control
- WB: Rat lung treated with lipopolysaccharides (LPS) culture supernatant.
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General notes
Ab229376 is the carrier-free version of ab189486. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab229376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18170-142 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 330122 Mouse
- Entrez Gene: 171551 Rat
- SwissProt: Q6W5C0 Mouse
- SwissProt: Q10746 Rat
- Unigene: 244289 Mouse
- Unigene: 10525 Rat
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Alternative names
- C-X-C motif chemokine 3 antibody
- C-X-C motif chemokine ligand 3 antibody
- Chemokine (C X C motif) ligand 3 antibody
see all
Images
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![Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-321746-anti-gro-gamma-antibody-epr18170-142-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
Lane 2: ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
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![Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-295228-anti-gro-gamma-antibody-epr18170-142-western-blot.jpg)
Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution
Lane 1 : Untreated rat lung culture supernatant
Lane 2 : Rat lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Exposure time: 6 secondsBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
The culture supernatant was collected from a large culture dish and it was not concentrated.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
Datasheets and documents
References
ab229376 has not yet been referenced specifically in any publications.
