Recombinant Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
![Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-321746-anti-gro-gamma-antibody-epr18170-142-immunoprecipitation.jpg "Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free
See all GRO gamma primary antibodies -
Description
Rabbit monoclonal [EPR18170-142] to GRO gamma - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6W5C0 -
Positive control
- WB: Rat lung treated with lipopolysaccharides (LPS) culture supernatant.
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General notes
Ab229376 is the carrier-free version of ab189486. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab229376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18170-142 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 330122 Mouse
- Entrez Gene: 171551 Rat
- SwissProt: Q6W5C0 Mouse
- SwissProt: Q10746 Rat
- Unigene: 244289 Mouse
- Unigene: 10525 Rat
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Alternative names
- C-X-C motif chemokine 3 antibody
- C-X-C motif chemokine ligand 3 antibody
- Chemokine (C X C motif) ligand 3 antibody
see all
Images
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Immunoprecipitation - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
GRO gamma was immunoprecipitated from 0.35 mg mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant with ab189486 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189486 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 10 μl (Input).
Lane 2: ab189486 IP in mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189486 in Mouse lung treated with 5 μg/ml lipopolysaccharides (LPS) for 6.6 days, culture supernatant 100 μl (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
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![Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-295228-anti-gro-gamma-antibody-epr18170-142-western-blot.jpg)
Western blot - Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)All lanes : Anti-GRO gamma antibody [EPR18170-142] (ab189486) at 1/1000 dilution
Lane 1 : Untreated rat lung culture supernatant
Lane 2 : Rat lung treated with 5 µg/ml lipopolysaccharides (LPS) for 6 days, culture supernatant
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Exposure time: 6 secondsBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
The culture supernatant was collected from a large culture dish and it was not concentrated.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189486).
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![Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)](https://www.abcam.com/ps/products/229/ab229376/Images/ab229376-3-benefits-of-recombinant-antibodies.png)
Anti-GRO gamma antibody [EPR18170-142] - BSA and Azide free (ab229376)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab229376 has not yet been referenced specifically in any publications.
