Overview

  • Product name

    Anti-GRO gamma antibody [EPR21759-11]
    See all GRO gamma primary antibodies
  • Description

    Rabbit monoclonal [EPR21759-11] to GRO gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein within Rat GRO gamma aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q10746

  • Positive control

    • WB: Rat CINC-2 recombinant protein (aa33-100); Rat lung primary cell supernatant; RAW264.7 treated with LPS for 6 hours, with BFA added after 3 hours, whole cell lysate. ICC/IF: RAW 264.7 cells treated with LPS for 6 hours, with BFA added after 3 hours. Flow Cyt: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with LPS for 6 hours, with BFA added after 3 hours. IP: RAW264.7 treated with LPS for 6 hours, with BFA(added after 3 hours, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21759-11
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab220431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ICC/IF 1/1000.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 3 antibody
    • C-X-C motif chemokine ligand 3 antibody
    • Chemokine (C X C motif) ligand 3 antibody
    • Chemokine (CXC motif) ligand 3 antibody
    • Cinc 2 antibody
    • CINC 2b antibody
    • Cinc2 antibody
    • CINC2b antibody
    • CXCL 3 antibody
    • Cxcl3 antibody
    • CXCL3_HUMAN antibody
    • Cytokine induced neutrophil chemoattractant 2 antibody
    • Dcip1 antibody
    • Dendritic cell inflammatory protein 1 antibody
    • Gm1960 antibody
    • GRO protein gamma antibody
    • GRO-gamma antibody
    • GRO-gamma(1-73) antibody
    • GRO-gamma(5-73) antibody
    • GRO3 antibody
    • GRO3 oncogene antibody
    • GROG antibody
    • Growth regulated protein gamma antibody
    • Growth-regulated protein gamma antibody
    • Macrophage inflammatory protein 2 beta precursor antibody
    • Macrophage inflammatory protein 2-beta antibody
    • Melanoma growth stimulatory activity gamma antibody
    • Member 3 antibody
    • MGSA gamma antibody
    • MIP 2b antibody
    • MIP2-beta antibody
    • MIP2B antibody
    • SCYB3 antibody
    • Small inducible cytokine subfamily B antibody
    see all

Images

  • All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

    Lane 1 : Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300ng/ml Brefeldin A (BFA) added after 3 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of CINC-2 increased LPS treatment of macrophages (PMID: 9576061).

    Note: CINC-2 and GRO gamma are alternative names for the same target.

  • GRO gamma was immunoprecipitated from 0.35 mg of RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate with ab220431 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220431 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate 10 µg (Input). 

    Lane 2: ab220431 IP in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220431 in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours (Right panel) or untreated (Left panel) labeling GRO gamma with ab220431 at 1/500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Note: CINC-2 and GRO gamma are alternative names for the same target.

  • All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

    Lane 1 : Rat CINC-2 recombinant protein (aa33-100) at 0.01 µg
    Lane 2 : Rat lung primary cell supernatant at 10 µl

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 11 kDa



    Exposure time : Lane 1: 15 seconds; Lane 2: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW : 9 kDa (lane 1), 11 kDa (lane 2).

    The molecular mass observed is consistent with what has been described in the literature (PMID: 12508094).

    Note: CINC-2 and GRO gamma are alternative names for the same target.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours or untreated labeling GRO gamma with ab220431 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

References

ab220431 has not yet been referenced specifically in any publications.

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