Recombinant
RabMAb

Recombinant Anti-GRO gamma antibody [EPR21759-11] (ab220431)

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Overview

  • Product name

    Anti-GRO gamma antibody [EPR21759-11]
    See all GRO gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR21759-11] to GRO gamma

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant full length protein within Rat GRO gamma aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q10746

  • Positive control

    • WB: Rat CINC-2 recombinant protein (aa33-100); Rat lung primary cell supernatant; RAW264.7 treated with LPS for 6 hours, with BFA added after 3 hours, whole cell lysate. ICC/IF: RAW 264.7 cells treated with LPS for 6 hours, with BFA added after 3 hours. Flow Cyt: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with LPS for 6 hours, with BFA added after 3 hours. IP: RAW264.7 treated with LPS for 6 hours, with BFA(added after 3 hours, whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21759-11

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab220431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ICC/IF 1/1000.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Post-translational
    modifications

    N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • C-X-C motif chemokine 3 antibody
      • C-X-C motif chemokine ligand 3 antibody
      • Chemokine (C X C motif) ligand 3 antibody
      • Chemokine (CXC motif) ligand 3 antibody
      • Cinc 2 antibody
      • CINC 2b antibody
      • Cinc2 antibody
      • CINC2b antibody
      • CXCL 3 antibody
      • Cxcl3 antibody
      • CXCL3_HUMAN antibody
      • Cytokine induced neutrophil chemoattractant 2 antibody
      • Dcip1 antibody
      • Dendritic cell inflammatory protein 1 antibody
      • Gm1960 antibody
      • GRO protein gamma antibody
      • GRO-gamma antibody
      • GRO-gamma(1-73) antibody
      • GRO-gamma(5-73) antibody
      • GRO3 antibody
      • GRO3 oncogene antibody
      • GROG antibody
      • Growth regulated protein gamma antibody
      • Growth-regulated protein gamma antibody
      • Macrophage inflammatory protein 2 beta precursor antibody
      • Macrophage inflammatory protein 2-beta antibody
      • Melanoma growth stimulatory activity gamma antibody
      • Member 3 antibody
      • MGSA gamma antibody
      • MIP 2b antibody
      • MIP2-beta antibody
      • MIP2B antibody
      • SCYB3 antibody
      • Small inducible cytokine subfamily B antibody
      see all

    Images

    • Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

      Lane 1 : Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 2 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300ng/ml Brefeldin A (BFA) added after 3 hours whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 3 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression of CINC-2 increased LPS treatment of macrophages (PMID: 9576061).

      Note: CINC-2 and GRO gamma are alternative names for the same target.

    • Immunoprecipitation - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      Immunoprecipitation - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

      GRO gamma was immunoprecipitated from 0.35 mg of RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate with ab220431 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220431 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate 10 µg (Input). 

      Lane 2: ab220431 IP in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220431 in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Flow Cytometry - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      Flow Cytometry - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours (Right panel) or untreated (Left panel) labeling GRO gamma with ab220431 at 1/500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Note: CINC-2 and GRO gamma are alternative names for the same target.

    • Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution

      Lane 1 : Rat CINC-2 recombinant protein (aa33-100) at 0.01 µg
      Lane 2 : Rat lung primary cell supernatant at 10 µl

      Secondary
      Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 11 kDa



      Exposure time : Lane 1: 15 seconds; Lane 2: 3 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Observed MW : 9 kDa (lane 1), 11 kDa (lane 2).

      The molecular mass observed is consistent with what has been described in the literature (PMID: 12508094).

      Note: CINC-2 and GRO gamma are alternative names for the same target.

    • Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (ab220431)
      Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (ab220431)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours or untreated labeling GRO gamma with ab220431 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    References

    ab220431 has not yet been referenced specifically in any publications.

    Publishing research using ab220431? Please let us know so that we can cite the reference in this datasheet.

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