Recombinant Anti-GRO gamma antibody [EPR21759-11] (ab220431)
![Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-311750-anti-gro-gamma-antibody-epr21759-11-western-blot.jpg "Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21759-11] to GRO gamma
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-GRO gamma antibody [EPR21759-11]
See all GRO gamma primary antibodies -
Description
Rabbit monoclonal [EPR21759-11] to GRO gamma -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein within Rat GRO gamma aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q10746 -
Positive control
- WB: Rat CINC-2 recombinant protein (aa33-100); Rat lung primary cell supernatant; RAW264.7 treated with LPS for 6 hours, with BFA added after 3 hours, whole cell lysate. ICC/IF: RAW 264.7 cells treated with LPS for 6 hours, with BFA added after 3 hours. Flow Cyt: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with LPS for 6 hours, with BFA added after 3 hours. IP: RAW264.7 treated with LPS for 6 hours, with BFA(added after 3 hours, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21759-11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab220431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). | |
| ICC/IF | 1/1000. | |
| Flow Cyt | 1/500. | |
| IP | 1/30. |
Target
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 330122 Mouse
- Entrez Gene: 171551 Rat
- SwissProt: Q6W5C0 Mouse
- SwissProt: Q10746 Rat
- Unigene: 244289 Mouse
- Unigene: 10525 Rat
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Alternative names
- C-X-C motif chemokine 3 antibody
- C-X-C motif chemokine ligand 3 antibody
- Chemokine (C X C motif) ligand 3 antibody
see all
Images
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Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution
Lane 1 : Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300ng/ml Brefeldin A (BFA) added after 3 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression of CINC-2 increased LPS treatment of macrophages (PMID: 9576061).
Note: CINC-2 and GRO gamma are alternative names for the same target.
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![Immunoprecipitation - Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-311752-anti-gro-gamma-antibody-epr21759-11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GRO gamma antibody [EPR21759-11] (ab220431)GRO gamma was immunoprecipitated from 0.35 mg of RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate with ab220431 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220431 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate 10 µg (Input).
Lane 2: ab220431 IP in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220431 in RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Flow Cytometry - Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-311751-anti-gro-gamma-antibody-epr21759-11-flow-cytometry.jpg)
Flow Cytometry - Anti-GRO gamma antibody [EPR21759-11] (ab220431)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours (Right panel) or untreated (Left panel) labeling GRO gamma with ab220431 at 1/500 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Note: CINC-2 and GRO gamma are alternative names for the same target.
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![Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-311749-anti-gro-gamma-antibody-epr21759-11-western-blot.jpg)
Western blot - Anti-GRO gamma antibody [EPR21759-11] (ab220431)All lanes : Anti-GRO gamma antibody [EPR21759-11] (ab220431) at 1/1000 dilution
Lane 1 : Rat CINC-2 recombinant protein (aa33-100) at 0.01 µg
Lane 2 : Rat lung primary cell supernatant at 10 µl
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 11 kDaExposure time : Lane 1: 15 seconds; Lane 2: 3 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Observed MW : 9 kDa (lane 1), 11 kDa (lane 2).
The molecular mass observed is consistent with what has been described in the literature (PMID: 12508094).
Note: CINC-2 and GRO gamma are alternative names for the same target.
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![Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-311748-anti-gro-gamma-antibody-epr21759-11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GRO gamma antibody [EPR21759-11] (ab220431)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours or untreated labeling GRO gamma with ab220431 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, with 300 ng/ml Brefeldin A (BFA) added after 3 hours.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-GRO gamma antibody [EPR21759-11] (ab220431)](https://www.abcam.com/ps/products/220/ab220431/Images/ab220431-6-benefits-of-recombinant-antibodies.png)
Anti-GRO gamma antibody [EPR21759-11] (ab220431)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab220431 has not yet been referenced specifically in any publications.
