Overview

  • Product name
    Anti-groEL antibody [9A1/2]
    See all groEL primary antibodies
  • Description
    Mouse monoclonal [9A1/2] to groEL
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Full length protein corresponding to Escherichia coli groEL.
    Database link: P0A6F5

  • Positive control
    • E. coli lysate.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab82592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IP Use at an assay dependent concentration.

Target

  • Relevance
    The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • 60 kDa chaperonin antibody
      • groEL protein antibody
      • groL antibody
      • mopA antibody
      • Protein Cpn60 antibody
      see all

    Images

    • All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution

      Lane 1 : groEL recombinant E. coli protein
      Lane 2 : Hsp60 recombinant human protein (negative control)
      Lane 3 : E. coli lysate

      Developed using the ECL technique.

      Predicted band size: 57 kDa
      Observed band size: 57 kDa



      This image was generated using the ascites version of the product.

    References

    This product has been referenced in:
    • Khodaparast L  et al. Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun 9:866 (2018). Read more (PubMed: 29491361) »
    • Lal A  et al. Regulation of Global Transcription in Escherichia coli by Rsd and 6S RNA. G3 (Bethesda) 8:2079-2089 (2018). Read more (PubMed: 29686109) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Answer


    I've looked through all of the information we have on this antibody, and unfortunately the epitope has not been mapped at this time.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up