Product nameAnti-groEL antibody [9A1/2]
See all groEL primary antibodies
DescriptionMouse monoclonal [9A1/2] to groEL
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Escherichia coli
Full length protein corresponding to Escherichia coli groEL.
Database link: P0A6F5
- E. coli lysate.
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
PurityTissue culture supernatant
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab82592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IP||Use at an assay dependent concentration.|
RelevanceThe bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution
Lane 1 : groEL recombinant E. coli protein
Lane 2 : Hsp60 recombinant human protein (negative control)
Lane 3 : E. coli lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
This image was generated using the ascites version of the product.
This product has been referenced in:
- Khodaparast L et al. Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun 9:866 (2018). Read more (PubMed: 29491361) »
- Lal A et al. Regulation of Global Transcription in Escherichia coli by Rsd and 6S RNA. G3 (Bethesda) 8:2079-2089 (2018). Read more (PubMed: 29686109) »